Difference between revisions of "PMID:14153532"
(Fill PMID: Page!) |
(→Notes) |
||
| (8 intermediate revisions by the same user not shown) | |||
| Line 35: | Line 35: | ||
==Main Points of the Paper == | ==Main Points of the Paper == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
| + | |||
| + | *Describes the introduction of mutations (by incorporation of 5BU into DNA) that results in cells unable to ferment lactose | ||
| + | *Sectoring phenotypes appear, indicative of the timing the mutation gets incorporated within a cell of a colony | ||
== Materials and Methods Used == | == Materials and Methods Used == | ||
{{LitMaterials}} | {{LitMaterials}} | ||
| + | |||
| + | *Parent strain: Z-15 (radiation-resistant, serine-requiring variant of the thymineless strain B3) | ||
| + | |||
| + | *[[:Category:5-Bromouracil Mutagenesis|5-Bromouracil Mutagenesis]] (on plates) | ||
| + | **strain plated on 5BU completed 1 round of replication, whereby most of the thymine was replaced by 5BU | ||
| + | **strain plated on thymine (control) completed a normal round of DNA replication | ||
| + | *Bacto-eosin-methylene blue (EMB) indicator agar (for more information, click [http://en.wikipedia.org/wiki/Eosin_methylene_blue here]) | ||
| + | **dark red colonies (lactose +) | ||
| + | **colorless colonies (lactose -) | ||
| + | **mixed | ||
| + | ***large sectors (lac- component originated at the center and constituted one-eighth or more the area of the colony) | ||
| + | ***peripheral sectors | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
| Line 51: | Line 66: | ||
|- | |- | ||
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
| + | |- | ||
| + | | | ||
| + | a mutation or genetic difference within a strain | ||
| + | | | ||
| + | *Taxon: Escherichia coli | ||
| + | *Strain: K-12 | ||
| + | *Substrain: z-15 | ||
| + | *NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333] | ||
| + | | | ||
| + | *Genotype of Reference Strain: Strain z-15 | ||
| + | *Genotype of Experimental Strain : Derivatives of Z-15 | ||
| + | | | ||
| + | *Reference Condition: | ||
| + | | | ||
| + | OMP:0000284 | ||
| + | | | ||
| + | loss of utilization of carbon-containing compound | ||
| + | | | ||
| + | ECO:0000178 | ||
| + | | | ||
| + | in vivo assay evidence | ||
| + | | | ||
| + | Abolished utilization of carbon source lactose in mutant strain. See Table 1. | ||
| + | | | ||
| + | |||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
| Line 58: | Line 98: | ||
==Notes== | ==Notes== | ||
| + | |||
| + | OMP: After carbon utilization terms get finialized, make sure OMP ID matches what is on here since it might change. | ||
| + | |||
| + | ECO: Terms for assays are awaiting implementation of solid and liquid child terms to each one. Experiment used EMB | ||
==References== | ==References== | ||
| Line 65: | Line 109: | ||
[[Category:Publication]] | [[Category:Publication]] | ||
| + | [[Category:5-Bromouracil Mutagenesis]] | ||
| + | [[Category:LaRossa citations that need review]] | ||
Latest revision as of 12:48, 19 April 2013
| Citation |
WITKIN, EM and SICURELLA, NA (1964) PURE CLONES OF LACTOSE-NEGATIVE MUTANTS OBTAINED IN ESCHERICHIA COLI AFTER TREATMENT WITH 5-BROMOURACIL. J. Mol. Biol. 8:610-3 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
|
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
- Describes the introduction of mutations (by incorporation of 5BU into DNA) that results in cells unable to ferment lactose
- Sectoring phenotypes appear, indicative of the timing the mutation gets incorporated within a cell of a colony
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- Parent strain: Z-15 (radiation-resistant, serine-requiring variant of the thymineless strain B3)
- 5-Bromouracil Mutagenesis (on plates)
- strain plated on 5BU completed 1 round of replication, whereby most of the thymine was replaced by 5BU
- strain plated on thymine (control) completed a normal round of DNA replication
- Bacto-eosin-methylene blue (EMB) indicator agar (for more information, click here)
- dark red colonies (lactose +)
- colorless colonies (lactose -)
- mixed
- large sectors (lac- component originated at the center and constituted one-eighth or more the area of the colony)
- peripheral sectors
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0000284 |
loss of utilization of carbon-containing compound |
ECO:0000178 |
in vivo assay evidence |
Abolished utilization of carbon source lactose in mutant strain. See Table 1. |
|
| edit table |
</protect>
Notes
OMP: After carbon utilization terms get finialized, make sure OMP ID matches what is on here since it might change.
ECO: Terms for assays are awaiting implementation of solid and liquid child terms to each one. Experiment used EMB
References
See Help:References for how to manage references in omp dev.
