Difference between revisions of "PMID:330501"

From omp dev
Jump to: navigation, search
(New PMID: Page!)
 
(→‎References)
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
 +
{{RightTOC}}
  
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.4071.D51ce134636d58-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="D51ce134636d58"  class=" tableEdit PMID_info_table" 
 +
 +
|-
 +
!align=left  |Citation
 +
||
 +
'''Hobson, AC, Gho, D and Müller-Hill, B'''  (1977) Isolation, genetic analysis, and characterization of Escherichia coli mutants with defects in the lacY gene. ''J. Bacteriol.'' '''131''':830-8
 +
|-
 +
!align=left  |Abstract
 +
||
 +
Five hundred thirty-five lacY mutants were isolated from an Escherichia coli strain carrying the lactose operon on an F' factor, either without mutagenesis or after mutagenesis with 2-aminopurine or N-methyl-N'-nitro-N-nitrosoguanidine. Crosses against 48 independently isolated deletions ending in the lacY gene divided the gene into 36 deletion groups. Suppressibility studies with 7 nonsense suppressor strains classified 276 mutants as nonsense mutants and 78 as missense (or nonsuppressible) mutants. One hundred seventy-nine mutants were "leaky" and could not be so allocated, and two were found to have small internal deletions. Nonsense mutants could in many cases be subdivided even within deletion groups on the basis of their suppressibility pattern, giving a total of 70 groups of nonsense mutants. Studies of these mutants allow the following conclusions: lactose and melibiose most probably do not have separate binding sites on the permease; the lacY region most likely consists of one cistron, and so both active transport and facilitated diffusion are functions of one protein; and finally, there is probably no small defined region of the permease responsible for energy coupling of transport. Furthermore, the strains and the analysis form the basis for a future functional study of the permease by biochemical techniques.
 +
|-
 +
!align=left  |Links
 +
||
 +
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=330501 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC235538 PMC235538]
 +
 +
|-
 +
!align=left  |Keywords
 +
||
 +
2-Aminopurine; Biological Transport, Active; Chromosome Mapping; Diffusion; Disaccharides/metabolism; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Galactosidases/metabolism; Genes; Lactose/metabolism; Membrane Transport Proteins/metabolism; Methylnitronitrosoguanidine; Mutagens; Mutation; Suppression, Genetic
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.4071.D51ce134636d58&page=4071&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.4071.D51ce134636d58-->
 +
 +
==Main Points of the Paper ==
 +
{{LitSignificance}}
 +
 +
== Materials and Methods Used ==
 +
{{LitMaterials}}
 +
 +
==Phenotype Annotations==
 +
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.4071.W51ce134674fa7-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="W51ce134674fa7"  class=" tableEdit Phenotype_Table_2" 
 +
|-
 +
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain:
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''lacY''
 +
*Genotype of Experimental Strain : ''lacY'' in strain MAD59-lacY
 +
|
 +
*Reference Condition: 42C
 +
*Experimental Condition: 42C
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
No growth when Malibose was sole carbon source at 42C.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain:
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''lacY''
 +
*Genotype of Experimental Strain : ''lacY'' in strain MAD59-lacY
 +
|
 +
*Reference Condition: 42C
 +
*Experimental Condition: 42C
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
 +
|
 +
No growth when Lactose was sole carbon source at 42C.
 +
|
 +
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.4071.W51ce134674fa7&page=4071&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.4071.W51ce134674fa7--></protect>
 +
 +
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 +
<references/>
 +
 +
 +
[[Category:Publication]]
 +
 +
[[Category:LaRossa citations that need review]]

Latest revision as of 18:41, 12 July 2013

Citation

Hobson, AC, Gho, D and Müller-Hill, B (1977) Isolation, genetic analysis, and characterization of Escherichia coli mutants with defects in the lacY gene. J. Bacteriol. 131:830-8

Abstract

Five hundred thirty-five lacY mutants were isolated from an Escherichia coli strain carrying the lactose operon on an F' factor, either without mutagenesis or after mutagenesis with 2-aminopurine or N-methyl-N'-nitro-N-nitrosoguanidine. Crosses against 48 independently isolated deletions ending in the lacY gene divided the gene into 36 deletion groups. Suppressibility studies with 7 nonsense suppressor strains classified 276 mutants as nonsense mutants and 78 as missense (or nonsuppressible) mutants. One hundred seventy-nine mutants were "leaky" and could not be so allocated, and two were found to have small internal deletions. Nonsense mutants could in many cases be subdivided even within deletion groups on the basis of their suppressibility pattern, giving a total of 70 groups of nonsense mutants. Studies of these mutants allow the following conclusions: lactose and melibiose most probably do not have separate binding sites on the permease; the lacY region most likely consists of one cistron, and so both active transport and facilitated diffusion are functions of one protein; and finally, there is probably no small defined region of the permease responsible for energy coupling of transport. Furthermore, the strains and the analysis form the basis for a future functional study of the permease by biochemical techniques.

Links

PubMed PMC235538

Keywords

2-Aminopurine; Biological Transport, Active; Chromosome Mapping; Diffusion; Disaccharides/metabolism; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Galactosidases/metabolism; Genes; Lactose/metabolism; Membrane Transport Proteins/metabolism; Methylnitronitrosoguanidine; Mutagens; Mutation; Suppression, Genetic

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: lacY
  • Genotype of Experimental Strain : lacY in strain MAD59-lacY
  • Reference Condition: 42C
  • Experimental Condition: 42C

No growth when Malibose was sole carbon source at 42C.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: lacY
  • Genotype of Experimental Strain : lacY in strain MAD59-lacY
  • Reference Condition: 42C
  • Experimental Condition: 42C

No growth when Lactose was sole carbon source at 42C.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:330501&oldid=10552"