Difference between revisions of "PMID:1931824"

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{|  id="Q4d768840b9b9c"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Blakely, G, Colloms, S, May, G, Burke, M and Sherratt, D'''  (1991) Escherichia coli XerC recombinase is required for chromosomal segregation at cell division.''New Biol.'' '''3''':789-98
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!align=left  |Abstract
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XerC is a site-specific recombinase of the bacteriophage lambda integrase family that is encoded by xerC at 3700 kbp on the genetic map of Escherichia coli. The protein was originally identified through its role in converting multimers of plasmid ColE1 to monomers; only monomers are stably inherited. Here we demonstrate that XerC also has a role in the segregation of replicated chromosomes at cell division. xerC mutants form filaments with aberrant nucleotides that appear unable to partition correctly. A DNA segment (dif) from the replication terminus region of the E. coli chromosome binds XerC and acts as a substrate for XerC-mediated site-specific recombination when inserted into multicopy plasmids. This dif segment contains a region of 28 bp with sequence similarity to the crossover region of ColE1 cer. The cell division phenotype of xerC mutants is suppressed in strains deficient in homologous recombination, suggesting that the role of XerC/dif in chromosomal metabolism is to convert any chromosomal multimers (arising through homologous recombination) to monomers.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1931824 PubMed]
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!align=left  |Keywords
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Site-specific Recombination; Chromosome Dimers; Resolution; Binding Sites; Cell Division; Escherichia coli; Integrases; ''dif''; ''cer''
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==Main Points of the Paper ==
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*''xerC'' insertion mutants have defects in cell division and chromosomal nucleoid segregation
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*mutations in ''recA'' suppress the ''xerC<sup>-</sup>'' phenotype
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*site-specific recombination locus ''dif'' is related to ''cer'' (alignments included)
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*''dif'' is a substrate for XerC-mediated site-specific recombination
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*XerC recombination at ''dif'' doesn't require ArgR or PepA
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== Materials and Methods Used ==
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*''xerC+'' plasmid complementation of the ''xerC'' mutant phenotype
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{|  id="H4d768840c621f"  class=" tableEdit PMID_Phenotype_table" 
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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xerC
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small colonies
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Growth
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on broth-containing plates
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Plating Assay
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''xerCY17'' and ''xerC2'' are both insertion mutations
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xerC
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slow recovery from stationary phase
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Growth
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Growth Curve
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''xerCY17'' and ''xerC2'' are both insertion mutations
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xerC
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varying exponential growth rate
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Growth
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day-to-day variation of 80-100% compared to isogenic xerC+ strain
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Growth Curve
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''xerCY17'' and ''xerC2'' are both insertion mutations
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xerC
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filamented cells
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Morphology
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variable number of filaments
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Microscopy
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''xerCY17'' and ''xerC2'' are both insertion mutations
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xerC
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nonfilamentous cells at the two-cell stage
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Morphology
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excess number of these within the population
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Microscopy
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''xerCY17'' and ''xerC2'' are both insertion mutations
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|-
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xerC
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mispositioned nucleoid
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Morphology
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single, highly amplified nucleoid at the filament midpoint and sometimes other places within the cell
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|
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Microscopy
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Fiigure 1-B2: ''xerCY17'' and ''xerC2'' are both insertion mutations
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|-
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xerC
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abolished DNA dimer resolution
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Growth
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|
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using a plasmid with ''dif'' site as a reporter for chromosome dimer resolution
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|
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Biochemical Assay
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|
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Figure 3- ''xerCY17'' and ''xerC2'' are both insertion mutations
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|- class="tableEdit_footer"
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]]
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[[Category:To Be Converted]]

Latest revision as of 12:09, 22 June 2011

Citation

Blakely, G, Colloms, S, May, G, Burke, M and Sherratt, D (1991) Escherichia coli XerC recombinase is required for chromosomal segregation at cell division.New Biol. 3:789-98

Abstract

XerC is a site-specific recombinase of the bacteriophage lambda integrase family that is encoded by xerC at 3700 kbp on the genetic map of Escherichia coli. The protein was originally identified through its role in converting multimers of plasmid ColE1 to monomers; only monomers are stably inherited. Here we demonstrate that XerC also has a role in the segregation of replicated chromosomes at cell division. xerC mutants form filaments with aberrant nucleotides that appear unable to partition correctly. A DNA segment (dif) from the replication terminus region of the E. coli chromosome binds XerC and acts as a substrate for XerC-mediated site-specific recombination when inserted into multicopy plasmids. This dif segment contains a region of 28 bp with sequence similarity to the crossover region of ColE1 cer. The cell division phenotype of xerC mutants is suppressed in strains deficient in homologous recombination, suggesting that the role of XerC/dif in chromosomal metabolism is to convert any chromosomal multimers (arising through homologous recombination) to monomers.

Links

PubMed

Keywords

Site-specific Recombination; Chromosome Dimers; Resolution; Binding Sites; Cell Division; Escherichia coli; Integrases; dif; cer

edit table

Main Points of the Paper

  • xerC insertion mutants have defects in cell division and chromosomal nucleoid segregation
  • mutations in recA suppress the xerC- phenotype
  • site-specific recombination locus dif is related to cer (alignments included)
  • dif is a substrate for XerC-mediated site-specific recombination
  • XerC recombination at dif doesn't require ArgR or PepA

Materials and Methods Used

  • xerC+ plasmid complementation of the xerC mutant phenotype

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

xerC

small colonies

Growth

on broth-containing plates

Plating Assay

xerCY17 and xerC2 are both insertion mutations

xerC

slow recovery from stationary phase

Growth

Growth Curve

xerCY17 and xerC2 are both insertion mutations

xerC

varying exponential growth rate

Growth

day-to-day variation of 80-100% compared to isogenic xerC+ strain

Growth Curve

xerCY17 and xerC2 are both insertion mutations

xerC

filamented cells

Morphology

variable number of filaments

Microscopy

xerCY17 and xerC2 are both insertion mutations

xerC

nonfilamentous cells at the two-cell stage

Morphology

excess number of these within the population

Microscopy

xerCY17 and xerC2 are both insertion mutations

xerC

mispositioned nucleoid

Morphology

single, highly amplified nucleoid at the filament midpoint and sometimes other places within the cell

Microscopy

Fiigure 1-B2: xerCY17 and xerC2 are both insertion mutations

xerC

abolished DNA dimer resolution

Growth

using a plasmid with dif site as a reporter for chromosome dimer resolution

Biochemical Assay

Figure 3- xerCY17 and xerC2 are both insertion mutations

edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:1931824&oldid=5145"