Difference between revisions of "PMID:19187760"

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'''Madabhushi, R and Marians, KJ'''  (2009) Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.''Mol. Cell'' '''33''':171-80
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!align=left  |Abstract
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In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this temporal regulation is unknown. Here we report that the bacterial actin homolog MreB participates in the temporal oscillation of Topo IV activity. We show that mreB mutant strains are deficient in Topo IV activity. In addition, we demonstrate that, depending upon whether it is in a monomeric or polymerized state, MreB affects Topo IV activity differentially. In addition, MreB physically interacts with the ParC subunit of Topo IV. Together, these results may explain how dynamics of the bacterial cytoskeleton are coordinated with the timing of chromosome segregation.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19187760 PubMed]
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Online version:[http://dx.doi.org/10.1016/j.molcel.2009.01.001 10.1016/j.molcel.2009.01.001]
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!align=left  |Keywords
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Actins; Binding Sites; Cell Cycle; Chromosome Segregation; Chromosomes, Bacterial; Cytoskeleton; DNA Topoisomerase IV; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Models, Biological; Mutation
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==Main Points of the Paper ==
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*MreB and TopoIV physically and functionally interact
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== Materials and Methods Used ==
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*Fluorescence microscopy
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==Phenotype Annotations==
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{|  id="U4d7e605f27471"  class=" tableEdit PMID_Phenotype_table" 
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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''Escherichia coli''
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PA340-678
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''mreB''
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increased size of nucleoid
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Morphology
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Microscopy
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Figure 1 and 2
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''Escherichia coli''
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PA340-678
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''mreB''
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decreased topoisomerase IV activity
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Morphology
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Microscopy
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Figure 1 and 2
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''Escherichia coli''
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NCBI:83333
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PA340
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dumbbell-shaped nucleoids
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Morphology
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Microscopy
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Figure 1 and 2
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|-
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''Escherichia coli''
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NCBI:83333
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PA340-678
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''mreB''
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increased number of singlet nucleoids
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Morphology
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Microscopy
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Figure 1 and 2
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]]
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[[Category:To Be Converted]]

Latest revision as of 12:09, 22 June 2011

Citation

Madabhushi, R and Marians, KJ (2009) Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.Mol. Cell 33:171-80

Abstract

In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this temporal regulation is unknown. Here we report that the bacterial actin homolog MreB participates in the temporal oscillation of Topo IV activity. We show that mreB mutant strains are deficient in Topo IV activity. In addition, we demonstrate that, depending upon whether it is in a monomeric or polymerized state, MreB affects Topo IV activity differentially. In addition, MreB physically interacts with the ParC subunit of Topo IV. Together, these results may explain how dynamics of the bacterial cytoskeleton are coordinated with the timing of chromosome segregation.

Links

PubMed Online version:10.1016/j.molcel.2009.01.001

Keywords

Actins; Binding Sites; Cell Cycle; Chromosome Segregation; Chromosomes, Bacterial; Cytoskeleton; DNA Topoisomerase IV; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Models, Biological; Mutation

edit table

Main Points of the Paper

  • MreB and TopoIV physically and functionally interact

Materials and Methods Used

  • Fluorescence microscopy

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

PA340-678

mreB

increased size of nucleoid

Morphology

Microscopy

Figure 1 and 2

Escherichia coli

PA340-678

mreB

decreased topoisomerase IV activity

Morphology

Microscopy

Figure 1 and 2

Escherichia coli

NCBI:83333

PA340

dumbbell-shaped nucleoids

Morphology

Microscopy

Figure 1 and 2

Escherichia coli

NCBI:83333

PA340-678

mreB

increased number of singlet nucleoids

Morphology

Microscopy

Figure 1 and 2

edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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