Difference between revisions of "PMID:4583216"

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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
 
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
MFT108
 +
|
 +
''ftsC''
 +
|
 +
*abolished septation at high temperature
 +
*temperature-sensitive increase in filamentation
 +
|
 +
Morphology
 +
|
 +
septation arrested immediately when the temperature of incubation was shifted from 30C to 41C
 +
|
 +
Microscopy
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
PAT84
 +
|
 +
''ftsA''
 +
|
 +
*decreased survival when grown at high temperature
 +
*temperature-sensitive decrease in survival
 +
|
 +
Growth
 +
|
 +
CFU counts decrease when cells are grown at 41C
 +
|
 +
Plating Assay
 +
|
 +
Figure 1A
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
MFT84
 +
|
 +
''ftsB''
 +
|
 +
*decreased survival when grown at high temperature
 +
*temperature-sensitive decreased survival
 +
|
 +
Growth
 +
|
 +
CFU counts decrease when cells are grown at 41C
 +
|
 +
Plating Assay
 +
|
 +
Figure 1B
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
PAT84
 +
|
 +
''ftsA''
 +
|
 +
*abolished septation at high temperature
 +
*temperature-sensitive increased filamentation
 +
|
 +
Morphology
 +
|
 +
septation arrested immediately when the temperature of incubation was shifted from 30C to 41C
 +
|
 +
Microscopy
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
MFT84
 +
|
 +
''ftsB''
 +
|
 +
*abolished septation at high temperature
 +
*temperature-sensitive increased filamentation
 +
|
 +
Morphology
 +
|
 +
septation arrested immediately when the temperature of incubation was shifted from 30C to 41C
 +
|
 +
Microscopy
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
MFT108
 +
|
 +
''ftsC''
 +
|
 +
*increased production of anucleate cells at high temperature
 +
*temperature-sensitive increase of anucleate cells
 +
|
 +
Morphology
 +
|
 +
residual septation occurring at the ends of the filaments
 +
|
 +
Microscopy
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
MFT84
 +
|
 +
''ftsB''
 +
|
 +
*increased production of anucleate cells at high temperature
 +
*temperature-sensitive increase of anucleate cells
 +
|
 +
Morphology
 +
|
 +
residual septation occurring at the ends of the filaments
 +
|
 +
Microscopy
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
MFT108
 +
|
 +
''ftsC''
 +
|
 +
*decreased rate of DNA synthesis at high temperature
 +
*temperature-sensitive decrease in DNA synthesis
 +
|
 +
Other
 +
|
 +
<sup>3</sup>H-thymidine incorporation
 +
|
 +
Biochemical Assay
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
MFT103
 +
|
 +
''ftsD''
 +
|
 +
*decreased rate DNA synthesis at high temperature
 +
*temperature-sensitive decrease in DNA synthesis
 +
|
 +
Other
 +
|
 +
<sup>3</sup>H-thymidine incorporation-decreased rate begins ~90 minutes after temperature shift
 +
|
 +
Biochemical Assay
 +
|
 +
Figure 2 top
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
MFT103
 +
|
 +
''ftsD''
 +
|
 +
*decreased rate of RNA synthesis at high temperature
 +
*temperature-sensitive decrease in RNA synthesis
 +
|
 +
Other
 +
|
 +
<sup>14</sup>C-uracil incorporation-decreased rate begins 40 minutes after temperature shift
 +
|
 +
Biochemical Assay
 +
|
 +
Figure 2 bottom
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
 +
|
 +
''ftsF''
 +
|
 +
*abolished septation at high temperature
 +
*temperature-sensitive increased filamentation
 +
|
 +
Morphology
 +
|
 +
septation arrested immediately when the temperature of incubation was shifted from 30C to 41C
 +
|
 +
Microscopy
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
 +
|
 +
''ftsG''
 +
|
 +
*abolished septation at high temperature
 +
*temperature-sensitive increased filamentation
 +
|
 +
Morphology
 +
|
 +
septation arrested immediately when the temperature of incubation was shifted from 30C to 41C
 +
|
 +
Microscopy
 +
|
 +
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
 +
|
 +
 +
|
 +
''ftsE''
 +
|
 +
*abolished septation at high temperature
 +
*temperature-sensitive increased filamentation
 +
|
 +
Morphology
 +
|
 +
septation arrested immediately when the temperature of incubation was shifted from 30C to 41C
 +
|
 +
Microscopy
 +
|
 +
  
 
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{{RefHelp}}
 
{{RefHelp}}
 
<references/>
 
<references/>
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[[Category:Publication]]
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[[Category:To Be Converted]]

Latest revision as of 12:09, 22 June 2011

Citation

Ricard, M and Hirota, Y (1973) Process of cellular division in Escherichia coli: physiological study on thermosensitive mutants defective in cell division.J. Bacteriol. 116:314-22

Abstract

Thermosensitive fts mutants of Escherichia coli belonging to seven previously identified genetic classes (ftsA to ftsG) were studied from a physiological standpoint. These mutants immediately stopped dividing and formed multinucleated filaments when the temperature was shifted to 41 C. Macromolecular syntheses (deoxyribonucleic acid), ribonucleic acid, cell mass, and murein) continued exponentially for at least 40 to 120 min. The number of surviving bacteria remained constant during the time of incubation, and this number began to decrease exponentially, as the rate of cell mass increase leveled off from the initial rate. The recovery of cell division at 30 C in these filamentous cells was studied after 60 min of incubation at 41 C. The existence of three types of mutants was shown. The ftsA and ftsE mutants resumed cell division without new protein synthesis; ftsD mutants resumed cell division only if new protein synthesis occured, while ftsB, C, F and G mutants did not resume cell division at all. No alteration in the cell envelope was detected by the method used here, although the ftsA, B, D, F and G mutations, in contrast with ftsC and E, caused an increased resistance to penicillin G. It was also shown that the recA mutation did not suppress the effect of the fts mutations and that none of the lysogenic fts mutants induced prophage multiplication while forming filaments. The effects of osmotic pressure and salts which rescue the mutant phenotype is described.

Links

PubMed

Keywords

Bacterial Proteins; Carbon Radioisotopes; Cell Division; Cell Wall; DNA, Bacterial; Escherichia coli; Fluorescence; Lysogeny; Mutation; Penicillin G; Penicillin Resistance; Peptidoglycan; RNA, Bacterial; Temperature; Thymidine; Tritium; Uracil

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

MFT108

ftsC

  • abolished septation at high temperature
  • temperature-sensitive increase in filamentation

Morphology

septation arrested immediately when the temperature of incubation was shifted from 30C to 41C

Microscopy

Escherichia coli

PAT84

ftsA

  • decreased survival when grown at high temperature
  • temperature-sensitive decrease in survival

Growth

CFU counts decrease when cells are grown at 41C

Plating Assay

Figure 1A

Escherichia coli

MFT84

ftsB

  • decreased survival when grown at high temperature
  • temperature-sensitive decreased survival

Growth

CFU counts decrease when cells are grown at 41C

Plating Assay

Figure 1B

Escherichia coli

PAT84

ftsA

  • abolished septation at high temperature
  • temperature-sensitive increased filamentation

Morphology

septation arrested immediately when the temperature of incubation was shifted from 30C to 41C

Microscopy

Escherichia coli

MFT84

ftsB

  • abolished septation at high temperature
  • temperature-sensitive increased filamentation

Morphology

septation arrested immediately when the temperature of incubation was shifted from 30C to 41C

Microscopy

Escherichia coli

MFT108

ftsC

  • increased production of anucleate cells at high temperature
  • temperature-sensitive increase of anucleate cells

Morphology

residual septation occurring at the ends of the filaments

Microscopy

Escherichia coli

MFT84

ftsB

  • increased production of anucleate cells at high temperature
  • temperature-sensitive increase of anucleate cells

Morphology

residual septation occurring at the ends of the filaments

Microscopy

Escherichia coli

MFT108

ftsC

  • decreased rate of DNA synthesis at high temperature
  • temperature-sensitive decrease in DNA synthesis

Other

3H-thymidine incorporation

Biochemical Assay

Escherichia coli

MFT103

ftsD

  • decreased rate DNA synthesis at high temperature
  • temperature-sensitive decrease in DNA synthesis

Other

3H-thymidine incorporation-decreased rate begins ~90 minutes after temperature shift

Biochemical Assay

Figure 2 top

Escherichia coli

MFT103

ftsD

  • decreased rate of RNA synthesis at high temperature
  • temperature-sensitive decrease in RNA synthesis

Other

14C-uracil incorporation-decreased rate begins 40 minutes after temperature shift

Biochemical Assay

Figure 2 bottom

Escherichia coli

ftsF

  • abolished septation at high temperature
  • temperature-sensitive increased filamentation

Morphology

septation arrested immediately when the temperature of incubation was shifted from 30C to 41C

Microscopy

Escherichia coli

ftsG

  • abolished septation at high temperature
  • temperature-sensitive increased filamentation

Morphology

septation arrested immediately when the temperature of incubation was shifted from 30C to 41C

Microscopy

Escherichia coli

ftsE

  • abolished septation at high temperature
  • temperature-sensitive increased filamentation

Morphology

septation arrested immediately when the temperature of incubation was shifted from 30C to 41C

Microscopy


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</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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