Difference between revisions of "PMID:1100857"

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'''Aswad, D and Koshland, DE'''  (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants.''J. Mol. Biol.'' '''97''':225-35
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'''Aswad, D and Koshland, DE Jr'''  (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants. ''J. Mol. Biol.'' '''97''':225-35
 
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!align=left |Abstract
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No abstract in PubMed
 
No abstract in PubMed
 
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1100857 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1100857 PubMed]
  
 
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!align=left |Keywords
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Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium; Species Specificity
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Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium/isolation & purification; Salmonella typhimurium/physiology; Species Specificity
  
 
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==Main Points of the Paper ==
 
==Main Points of the Paper ==
 
{{LitSignificance}}
 
{{LitSignificance}}
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* Isolation of non-chemotactic mutants
 
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* Description of the preformed liquid gradient technique
 
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**utilizes a preformed gradient in a vertical column of liquid medium
 
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**separates wild-type, non-motile, and non-motile but chemotactic into 3 regions
 
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* None of the 72 mutants isolated exhibited a frequency of spontaneous tumbling
 
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**68 exhibited smooth co-ordinated motility with no evidence of tumbling
 
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**4 exhibited uncoordinated motility
 
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* Results suggest there may be as many as 6 genetically distinct classes of che mutants in Salmonella
 
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
 
{{LitMaterials}}
 
{{LitMaterials}}
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* For the isolation of non-chemotactic mutants
 
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**preformed liquid gradient technique (described in detail)
 
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**Diethylsulfate mutagenesis
 
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**negative swarm selection on Tryptone semi-solid medium (0.3% agar)
 
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#culture spotted on plate
 
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#overnight incubation (wt bacteria swarmed outward from the inoculum, leaving a dense population at the center)
 
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#sample taken from the center of colony
 
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#perform 10 such serial selections
 
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#grow samples in nutrient broth and plate on Tryptone semisolid medium
 
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#200 non-swarming colonies were picked and screened for motility by examination with a microscope
 
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*For complementations and strain constructions
 
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**Alternative method of abortive transduction
 
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*Strains
 
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**all strains were derived from ''Salmonella typhimurium'' LT2
 
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST20
 
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Motility
 
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smooth motility with no spontaneous tumbling
 
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Microscopy
 
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isolated from using negative swarm selection described above. Phenotype labeled ''che-222(p)''
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST20
 
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Motility
 
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consistently diffuses in the direction of gravity, regardless of attractant
 
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Other
 
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Preformed liquid gradient technique- Phenotype for this strain is labeled ''che-222(p)''
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST1
 
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Motility
 
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moves in the direction of attractant
 
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Other
 
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Preformed liquid gradient technique
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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TA1859
 
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Motility
 
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no significant movement in either direction
 
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Other
 
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Preformed liquid gradient technique.  This is a non-motile mutant strain.
 
  
 
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==Notes==
 
==Notes==
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==References==
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Controls for the preformed liquid gradient method were:
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#ST20 (non-chemotactic mutant strain described above)
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#TA1859 (non-motile mutant)
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[[Category:Publication]]
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#ST1 (wild type strain)
 

Latest revision as of 05:26, 26 July 2018

Citation

Aswad, D and Koshland, DE Jr (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants. J. Mol. Biol. 97:225-35

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium/isolation & purification; Salmonella typhimurium/physiology; Species Specificity

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:1100857&oldid=129230"