Difference between revisions of "PMID:1100857"

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'''Aswad, D and Koshland, DE'''  (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants.''J. Mol. Biol.'' '''97''':225-35
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'''Aswad, D and Koshland, DE Jr'''  (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants. ''J. Mol. Biol.'' '''97''':225-35
 
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!align=left |Abstract
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!align=left align='left' bgcolor='#CCCCFF' |Abstract
 
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No abstract in PubMed
 
No abstract in PubMed
 
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1100857 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1100857 PubMed]
  
 
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!align=left |Keywords
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Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium; Diethylsulfate mutagenesis; Preformed liquid gradient technique
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Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium/isolation & purification; Salmonella typhimurium/physiology; Species Specificity
  
 
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==Main Points of the Paper ==
 
==Main Points of the Paper ==
 
{{LitSignificance}}
 
{{LitSignificance}}
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* Isolation of non-chemotactic mutants
 
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* Description of the preformed liquid gradient technique
 
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**utilizes a preformed gradient in a vertical column of liquid medium
 
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**separates wild-type, non-motile, and non-motile but chemotactic into 3 regions
 
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* 72 mutants isolated
 
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**68 exhibited smooth co-ordinated motility with no evidence of tumbling
 
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**4 exhibited uncoordinated motility
 
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* Results suggest there may be as many as 6 genetically distinct classes of ''che'' mutants in ''Salmonella''
 
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
 
{{LitMaterials}}
 
{{LitMaterials}}
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* For the isolation of non-chemotactic mutants
 
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**Diethylsulfate mutagenesis
 
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**Negative swarm selection on Tryptone semi-solid medium (0.3% agar)
 
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**Preformed liquid gradient technique (described in detail)
 
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*For complementations and strain constructions
 
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**Alternative method of abortive transduction
 
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*Strains
 
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**all strains were derived from ''Salmonella typhimurium'' LT2
 
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**Initial cultures ST2 and ST23 (wt chemotaxis) were both mutagenized with diethylsulfate to generate mutants
 
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*For the initial ST2 culture
 
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#ST2 culture was mutagenized with diethylsulfate
 
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#culture spotted on plate
 
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#overnight incubation (wt bacteria swarmed outward from the inoculum, leaving a dense population at the center)
 
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#sample taken from the center of colony
 
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#perform 10 such serial selections
 
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#grow samples in nutrient broth and plate on Tryptone semisolid medium
 
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#200 non-swarming colonies were picked and screened for motility by examination with a microscope
 
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*For the ST23 culture
 
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#ST23 (wt chemotaxis) was mutagenized with diethylsulfate
 
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#''che'' mutants enriched in the preformed liquid gradient apparatus 4 times, allowing for turbid growth between each enrichment
 
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#final culture plated on tryptone semisolid medium to screen for non-swarming colonies
 
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3 distinct colony morphologies arose: (1)large colonies (wt); (2)tiny, compact colonies with sharp boundaries (non-motile); (3)tiny, compact colonies with fuzzy boundaries (''che'')
 
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST20
 
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*Positive gravitropism regardless of attractant
 
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*no response to gradient
 
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Motility
 
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consistently diffuses in the direction of gravity, regardless of attractant
 
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Other
 
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Preformed liquid gradient technique. Strain can also be labeled ST2''che-222(p)''
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST20
 
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*Smooth motility with no spontaneous tumbling
 
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Motility
 
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smooth motility with no spontaneous tumbling
 
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Microscopy
 
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isolated from using negative swarm selection described above. Phenotype labeled ''che-222(p)''
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST1
 
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*Wild-type chemotaxis
 
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*Positive response to gradient (attractant)
 
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Motility
 
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moves in the direction of attractant
 
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Other
 
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Preformed liquid gradient technique
 
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|-
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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TA1859
 
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*No response to gradient
 
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*Absent cell motility
 
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Motility
 
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no significant response to gradient
 
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Other
 
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Preformed liquid gradient technique.  This is a non-motile mutant strain.
 
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|-
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST23''che-223(e)''
 
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*Smooth motility in response to temporal gradient (serine)
 
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*Positive chemotaxis (wild-type chemotaxis?)
 
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Motility
 
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smooth motility in response to a temporal gradient of serine.
 
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Microscopy
 
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stroboscopic light source in dark field with an exposure time of 4s.
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST23''che-223(e)''
 
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*Uncoordinated motility
 
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Motility
 
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uncoordinated motility in either minimal medium or nutrient broth
 
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Microscopy
 
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stroboscopic light source in dark field with an exposure time of 4s.
 
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|-
 
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''Salmonella typhimurium'' LT2
 
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NCBI:99287
 
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ST23
 
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*Positive chemotaxis (towards attractant)
 
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*Wild-type chemotaxis
 
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Motility
 
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wt chemotaxis
 
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==Notes==
 
==Notes==
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==References==
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{{RefHelp}}
 
<references/>
 
<references/>
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*Controls for the first preformed liquid gradient experiment described in the paper were:
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#ST20
 
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#TA1859 (non-motile mutant)
 
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#ST1 (wild type strain)
 
  
 
[[Category:Publication]]
 
[[Category:Publication]]
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[[Category:To Be Converted]]
 

Latest revision as of 05:26, 26 July 2018

Citation

Aswad, D and Koshland, DE Jr (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants. J. Mol. Biol. 97:225-35

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium/isolation & purification; Salmonella typhimurium/physiology; Species Specificity

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:1100857&oldid=129230"