Difference between revisions of "PMID:14430363"

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'''PARDEE, AB and PRESTIDGE, LS'''  (1959) On the nature of the repressor of beta-galactosidase synthesis in Escherichia coli.''Biochim. Biophys. Acta'' '''36''':545-7
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'''No author listed'''  (1959) On the nature of the repressor of beta-galactosidase synthesis in Escherichia coli. ''Biochim. Biophys. Acta'' '''36''':545-7
 
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No abstract in PubMed
 
No abstract in PubMed
 
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14430363 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14430363 PubMed]
  
 
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!align=left |Keywords
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Escherichia coli; Glycoside Hydrolases
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Escherichia coli/metabolism; Glycoside Hydrolases/antagonists & inhibitors
  
 
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==Main Points of the Paper ==
 
==Main Points of the Paper ==
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*Hfr(''z<sup>+</sup>i<sup>+</sup>'')  X  F<sup>-</sup>(''z<sup>-</sup>i<sup>-</sup>''):
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{{LitSignificance}}
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**within a few minutes of the time of entry of the ''z<sup>+</sup>i<sup>+</sup>'' genes (which are closely linked) the synthesis of β-galactosidase commenced at maximal velocity in the absence of extracellular inducer
 
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**inducible i<sup>+</sup> allele appears dominant over the constitutive i<sup>-</sup> allele
 
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*The idea that i<sup>+</sup> could control the synthesis of a destructive enzyme which removes an endogenous intracellular inducer was eliminated due to observations from this paper (Figure 1, Curves C & D).
 
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*The presence of 5-methyltryptophan enhances mating efficiency
 
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*The data in this paper may suggest that:
 
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**an inducer-destroying enzyme is not responsible for the difference between inducibility and constitutivity
 
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**the repressor is not a protein, although the authors state this conclusion is open to doubt (evidence to refute this conclusion is shown [[PMID:5330222|here]]).
 
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
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*Strains:
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**Hfr (''z<sup>+</sup>i<sup>+</sup>)
 
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**F<sup>-</sup> (z<sup>-</sup>i<sup>-</sup>)
 
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*Conjugation
 
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*Reagents
 
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**Streptomycin
 
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**Chloramphenicol
 
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**5-methytryptophan
 
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**Duponol C(Na lauryl sulfate)
 
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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''Escherichia coli''
 
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NCBI:562
 
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lacZ- lacI-
 
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*Constitutive expression of beta-galactosidase
 
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Expression
 
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lac genes were transferred from Hfr (z+i+) into F- (z-i-) during conjugation and LacZ production became constitutive within the first few minutes after transfer
 
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Biochemical Assay
 
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Figure 1- curves A and B (LacZ assay)
 
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''Escherichia coli''
 
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NCBI:562
 
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lacZ- lacI-
 
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*Inducible expression of beta-galactosidase (wt phenotype)
 
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*Regulation of lacZ gene expression
 
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*Repression of lacZ gene expression
 
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Expression
 
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lac genes were transferred from Hfr (z+i+) into F- (z-i-) during conjugation and LacZ production became inducible about an hour after transfer
 
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Biochemical Assay
 
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Figure 1- curve A vs. curve B (LacZ assay)
 
  
 
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==Notes==
 
==Notes==
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==References==
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[[Category:Publication]]
 
[[Category:Publication]]
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[[Category:To Be Converted]]
 

Latest revision as of 13:16, 1 September 2013

Citation

No author listed (1959) On the nature of the repressor of beta-galactosidase synthesis in Escherichia coli. Biochim. Biophys. Acta 36:545-7

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Escherichia coli/metabolism; Glycoside Hydrolases/antagonists & inhibitors

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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