Difference between revisions of "PMID:4886277"

From omp dev
Jump to: navigation, search
(Table edited by Azweifel via TableEdit)
(Table edited by Siegele via TableEdit)
 
(5 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 
{{RightTOC}}
 
{{RightTOC}}
−
 
+
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.95.H5223848768024-->
−
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.95.A4cb8d709bef43-->
 
 
<!--
 
<!--
 
******************************************************************************************
 
******************************************************************************************
Line 8: Line 7:
 
*  
 
*  
 
****************************************************************************************** -->
 
****************************************************************************************** -->
−
{|  id="A4cb8d709bef43"  class=" tableEdit PMID_info_table"   
+
{|  id="H5223848768024"  class=" tableEdit PMID_info_table"   
  
 
|-
 
|-
−
!align=left |citation
+
!align=left align='left' bgcolor='#CCCCFF' |Citation
 
||
 
||
−
'''Squires, CK and Ingraham, JL'''  (1969) Mutant of Escherichia coli exhibiting a cold-sensitive phenotype for growth on lactose.''J. Bacteriol.'' '''97''':488-94
+
'''Squires, CK and Ingraham, JL'''  (1969) Mutant of Escherichia coli exhibiting a cold-sensitive phenotype for growth on lactose. ''J. Bacteriol.'' '''97''':488-94
 
|-
 
|-
−
!align=left |abstract
+
!align=left align='left' bgcolor='#CCCCFF' |Abstract
 
||
 
||
 
As part of a study on the effect of low temperature on cellular regulatory processes, a class of lactose-negative mutants of Escherichia coli K-12 was isolated which could use lactose as a sole carbon and energy source at 37 C, but which could not use this sugar at 20 C. The lactose operon of the mutants functioned normally at 20 C. Galactose exhibited a strong inhibitory effect on growth, especially at 20 C. Growth of the mutants on glycerol was stopped at 20 C and slowed considerably at 37 C if galactose was added to the medium. Making the mutants galactose-positive eliminated the cold sensitivity of lactose utilization. One mutant was shown to be galactose-1-phosphate uridyl transferase-negative, galactose-kinase heat-sensitive, and uridine diphosphate-galactose-4-epimerase-positive. It is postulated that the mutant is able to phosphorylate galactose at 20 C (if only at a very low rate), but lacking transferase it is poisoned by the accumulation of galactose-1-phosphate. At 37 C, galactokinase is nonfunctional and the mutant grows on the glucose moiety of lactose.
 
As part of a study on the effect of low temperature on cellular regulatory processes, a class of lactose-negative mutants of Escherichia coli K-12 was isolated which could use lactose as a sole carbon and energy source at 37 C, but which could not use this sugar at 20 C. The lactose operon of the mutants functioned normally at 20 C. Galactose exhibited a strong inhibitory effect on growth, especially at 20 C. Growth of the mutants on glycerol was stopped at 20 C and slowed considerably at 37 C if galactose was added to the medium. Making the mutants galactose-positive eliminated the cold sensitivity of lactose utilization. One mutant was shown to be galactose-1-phosphate uridyl transferase-negative, galactose-kinase heat-sensitive, and uridine diphosphate-galactose-4-epimerase-positive. It is postulated that the mutant is able to phosphorylate galactose at 20 C (if only at a very low rate), but lacking transferase it is poisoned by the accumulation of galactose-1-phosphate. At 37 C, galactokinase is nonfunctional and the mutant grows on the glucose moiety of lactose.
 
|-
 
|-
−
!align=left |links
+
!align=left align='left' bgcolor='#CCCCFF' |Links
 
||
 
||
−
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4886277 PubMed]
+
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4886277 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC249716 PMC249716]
  
 
|-
 
|-
−
!align=left |keywords
+
!align=left align='left' bgcolor='#CCCCFF' |Keywords
 
||
 
||
−
Enzyme Induction; Escherichia coli; Galactose; Galactosidases; Genetic Complementation Test; Genetics, Microbial; Glucose; Lactose; Membrane Transport Proteins; Molecular Biology; Mutation; Phosphotransferases; Temperature
+
Enzyme Induction; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/metabolism; Galactose/pharmacology; Galactosidases/metabolism; Genetic Complementation Test; Genetics, Microbial; Glucose/metabolism; Lactose/metabolism; Membrane Transport Proteins/metabolism; Molecular Biology; Mutation; Phosphotransferases/metabolism; Temperature
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  
−
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.95.A4cb8d709bef43&page=95&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
+
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.95.H5223848768024&page=95&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 
|}
 
|}
−
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.95.A4cb8d709bef43-->
+
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.95.H5223848768024-->
−
 
 
 
==Main Points of the Paper ==
 
==Main Points of the Paper ==
−
* Complementation of the ''gal'' genes is discussed
+
{{LitSignificance}}
−
**making the mutant ''gal<sup>+</sup>'' corrects the cold sensitivity of lactose fermentation and overcomes the toxic response to galactose at low temperatures
 
  
−
== Methods Used ==
+
== Materials and Methods Used ==
−
*Parent strain: KG531-1 (''his<sup>-</sup>, met<sup>-</sup>, thi<sup>-</sup>, galT<sup>-</sup>, strR, λ<sup>R</sup>'')
+
{{LitMaterials}}
−
*2-aminopurine (2-AP) mutagenesis
 
−
*β-galactosidase assay
 
−
*Galactosidase permease assay (uptake and hydrolysis of ONPG)
 
−
*Galactokinase assay
 
−
*P1 transductions
 
  
−
==Annotations==
+
==Phenotype Annotations==
−
<protect><!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.95.Z4cb8d709c022e-->
+
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.95.Z52238487e9a5f-->
 
<!--
 
<!--
 
******************************************************************************************
 
******************************************************************************************
Line 53: Line 46:
 
*  
 
*  
 
****************************************************************************************** -->
 
****************************************************************************************** -->
−
{|   id="Z4cb8d709c022e"  class=" tableEdit PMID_Phenotype_table"   
+
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z52238487e9a5f"  class=" tableEdit Phenotype_Table_2"   
−
|-
+
|- align='left' bgcolor='#CCCCFF'
−
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
+
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
−
|-
 
−
|
 
−
''Escherichia coli''
 
−
|
 
−
NCBI:562
 
−
|
 
−
K-III-4
 
−
|
 
−
 
 
−
|
 
−
cold-sensitive for growth on lactose-minimal plates
 
−
|
 
−
Growth
 
−
|
 
−
normal growth rates at 20C on glucose and at 37C on both glucose- and lactose- minimal media
 
−
|
 
−
Other
 
−
|
 
−
Table 2-Growth curve
 
−
|-
 
−
|
 
−
''Escherichia coli''
 
−
|
 
−
NCBI:562
 
−
|
 
−
K-III-4
 
−
|
 
−
 
 
−
|
 
−
decrease synthesis of beta-gal
 
−
|
 
−
Metabolic Activity
 
−
|
 
−
differential rate of beta-galactosidase synthesis fell to a very low value shortly before the mass had doubled.
 
−
|
 
−
Biochemical Assay
 
−
|
 
−
Figure 1-LacZ assay vs. dry weight
 
−
|-
 
−
|
 
−
''Escherichia coli''
 
−
|
 
−
NCBI:562
 
−
|
 
−
K-III-4
 
−
|
 
−
 
 
−
|
 
−
galactose-dependent toxicity at 20C and 37C (inhibition of growth)
 
−
|
 
−
Growth
 
−
|
 
−
the addition of galactose alone inhibited the growth of the mutant at both temperatures.
 
−
|
 
−
Other
 
−
|
 
−
Figure 2 and Table 6- Growth on galactose vs. lactose vs. glycerol at various temperatures
 
−
|-
 
−
|
 
−
''Escherichia coli''
 
−
|
 
−
NCBI:562
 
−
|
 
−
K-III-4
 
−
|
 
−
''galK''
 
−
|
 
−
ts for galactokinase at 37C
 
−
 
 
−
|
 
−
Metabolic Activity
 
−
|
 
−
galactokinase-negative at 37C, but positive at 20C
 
−
|
 
−
Biochemical Assay
 
−
|
 
−
Tables 7 and 8
 
−
|-
 
−
|
 
−
''Escherichia coli''
 
−
|
 
−
NCBI:562
 
−
|
 
−
K-III-4
 
−
|
 
−
''galT''
 
−
|
 
−
Cs for galactose-1-phosphate uridyl transferase at 20C
 
−
 
 
−
|
 
−
Metabolic Activity
 
−
|
 
−
galactose-1-phosphate uridyl transferase-negative at 20C, but positive at 37C
 
−
|
 
−
Biochemical Assay
 
−
|
 
−
Table 7- results of complementation tests
 
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  
−
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.95.Z4cb8d709c022e&page=95&pagename={{FULLPAGENAMEE}}&type=0&template=PMID_Phenotype_table edit table]</span> || || || || || || || ||
+
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.95.Z52238487e9a5f&page=95&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 
|}
 
|}
−
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.95.Z4cb8d709c022e--></protect>
+
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.95.Z52238487e9a5f--></protect>
  
 
==Notes==
 
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 
<references/>
 
<references/>
 +
 +
 +
[[Category:Publication]]

Latest revision as of 17:01, 29 November 2013

Citation

Squires, CK and Ingraham, JL (1969) Mutant of Escherichia coli exhibiting a cold-sensitive phenotype for growth on lactose. J. Bacteriol. 97:488-94

Abstract

As part of a study on the effect of low temperature on cellular regulatory processes, a class of lactose-negative mutants of Escherichia coli K-12 was isolated which could use lactose as a sole carbon and energy source at 37 C, but which could not use this sugar at 20 C. The lactose operon of the mutants functioned normally at 20 C. Galactose exhibited a strong inhibitory effect on growth, especially at 20 C. Growth of the mutants on glycerol was stopped at 20 C and slowed considerably at 37 C if galactose was added to the medium. Making the mutants galactose-positive eliminated the cold sensitivity of lactose utilization. One mutant was shown to be galactose-1-phosphate uridyl transferase-negative, galactose-kinase heat-sensitive, and uridine diphosphate-galactose-4-epimerase-positive. It is postulated that the mutant is able to phosphorylate galactose at 20 C (if only at a very low rate), but lacking transferase it is poisoned by the accumulation of galactose-1-phosphate. At 37 C, galactokinase is nonfunctional and the mutant grows on the glucose moiety of lactose.

Links

PubMed PMC249716

Keywords

Enzyme Induction; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/metabolism; Galactose/pharmacology; Galactosidases/metabolism; Genetic Complementation Test; Genetics, Microbial; Glucose/metabolism; Lactose/metabolism; Membrane Transport Proteins/metabolism; Molecular Biology; Mutation; Phosphotransferases/metabolism; Temperature

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:4886277&oldid=11138"