PMID:6363412

Citation	Davis, EO, Jones-Mortimer, MC and Henderson, PJ (1984) Location of a structural gene for xylose-H+ symport at 91 min on the linkage map of Escherichia coli K12.J. Biol. Chem. 259:1520-5
Abstract	Mutations in the xylose-H+ transport activity of Escherichia coli K12 were isolated using Mud(ApRlac). The initial selection was for simultaneous acquisition of ampicillin and xylose resistance in an fda background. Colonies were then screened for xylose-inducible beta-galactosidase and for growth on xylose of their fda+ derivatives. Two of the xylose-positive derivatives were shown to be impaired in xylose-H+ symport in whole cells and in xylose transport into subcellular vesicles. Their xylose transport in whole cells showed increased sensitivity to arsenate. The site of prophage insertion was mapped to 91.4 min on the E. coli genome between pgi and malB. It is proposed that the gene for the xylose-H+ symport system be called xylE.
Links	PubMed
Keywords	Biological Transport; Carrier Proteins; Chromosome Deletion; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli; Escherichia coli Proteins; Genes; Genes, Bacterial; Genetic Linkage; Genotype; Kinetics; Lactose; Mutation; Symporters; Transduction, Genetic; Xylose
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JM2087 NCBI Taxon ID: 83333	Genotype of Reference Strain: xylE+ Genotype of Experimental Strain : xylE mutation in strain JM2336	Reference Condition:	0000276	increased resistance to antibiotics	0000181	in vitro assay evidence	Mud(ApRlac)
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