Difference between revisions of "PMID:4381707"
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| + | {| id="L505798785c68f" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Bertino, JB and Stacey, KA ''' (1966) A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli. ''Biochem. J.'' '''101''':32C-33C | ||
| + | |- | ||
| + | !align=left |Abstract | ||
| + | || | ||
| + | No abstract in PubMed | ||
| + | |- | ||
| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4381707 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1270145 PMC1270145] | ||
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| + | !align=left |Keywords | ||
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| + | Anti-Bacterial Agents/pharmacology; Escherichia coli/enzymology; Ligases/metabolism; Molecular Biology; Mutation; Nucleotides; Streptococcus pneumoniae; Tetrahydrofolate Dehydrogenase/metabolism; Thymine | ||
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| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3499.L505798785c68f--> | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | {| id="Q5057987874ca8" class=" tableEdit Phenotype_Table_2" | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
| + | *It was found that an altered dihydrofolate can not be adduced wild type ''thy<sup>+</sup>'' and ''thy<sup>-</sup>'' extracts were examined. It was found that that the ''K<sub>m</sub>'' values for both their dihydrofolate-reductase activity was nearly the same (figure 1) as where the effects of KCl, CaCl<sub>2</sub> and other organic mercurials (Table 1) as was the overall activity levels of dihydrofolaate-reductase. Serine hydroxymethylase and ''N''<sup>5</sup>''N''<sup>10</sup>-methylene dehydroxgenase activities were also similar between the wild type and the mutant. | ||
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| + | *The correlation between the loss of thymidylate synthetase and resistance to inhibitors of dihydrofolate reductase may be due to the one striking difference between thymidylate synthetase. | ||
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| + | *''thy<sup>-</sup> mutants 15T<sup>-</sup> and B3 were found to be resistant to trimethoprim though 15T<sup>-</sup> was found to more resistant, the main difference between them being that 15T<sup>-</sup> has no thymidylate synthetase activity while B3 had at least 20 percent of the normal activity. | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
| + | |||
| + | |||
| + | [[Category:Publication]] | ||
| + | [[Category:Papers referenced in the LaRossa chapter]] | ||
Latest revision as of 17:18, 27 September 2012
| Citation |
Bertino, JB and Stacey, KA (1966) A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli. Biochem. J. 101:32C-33C |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Anti-Bacterial Agents/pharmacology; Escherichia coli/enzymology; Ligases/metabolism; Molecular Biology; Mutation; Nucleotides; Streptococcus pneumoniae; Tetrahydrofolate Dehydrogenase/metabolism; Thymine |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
- It was found that an altered dihydrofolate can not be adduced wild type thy+ and thy- extracts were examined. It was found that that the Km values for both their dihydrofolate-reductase activity was nearly the same (figure 1) as where the effects of KCl, CaCl2 and other organic mercurials (Table 1) as was the overall activity levels of dihydrofolaate-reductase. Serine hydroxymethylase and N5N10-methylene dehydroxgenase activities were also similar between the wild type and the mutant.
- The correlation between the loss of thymidylate synthetase and resistance to inhibitors of dihydrofolate reductase may be due to the one striking difference between thymidylate synthetase.
- thy- mutants 15T- and B3 were found to be resistant to trimethoprim though 15T- was found to more resistant, the main difference between them being that 15T- has no thymidylate synthetase activity while B3 had at least 20 percent of the normal activity.
References
See Help:References for how to manage references in omp dev.
