Difference between revisions of "PMID:4588694"

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{|  id="X5058e3e108452"  class=" tableEdit PMID_info_table" 
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|-
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!align=left  |Citation
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'''Kennedy, EP , Rumley, MK  and Armstrong, JB '''  (1974) Dierect measurement of the binding of labeled sugars to the lactose permease M protein. ''J. Biol. Chem.'' '''249''':33-7
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!align=left  |Abstract
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No abstract in PubMed
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4588694 PubMed]
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!align=left  |Keywords
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Bacterial Proteins/metabolism; Cell Membrane/drug effects; Cell Membrane/enzymology; Cell-Free System; Chromatography, Ion Exchange; Escherichia coli/cytology; Escherichia coli/enzymology; Galactose; Hydrogen-Ion Concentration; Isotope Labeling; Kinetics; Lactose; Membrane Transport Modulators; Membrane Transport Proteins/antagonists & inhibitors; Membrane Transport Proteins/metabolism; Methods; Mutation; Phosphorus Radioisotopes; Receptors, Drug; Structure-Activity Relationship; Surface-Active Agents/pharmacology; Thioglycosides/metabolism; Time Factors; Tritium
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==Main Points of the Paper ==
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{{LitSignificance}}
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*Describes a method for measuring the binding of labeled sugars to M (LacY) directly
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== Materials and Methods Used ==
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{{LitMaterials}}
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*Strain: A-324 (pro-lac i-z+y+F-)
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*Strain: A-3244 (pro-lac i-z+y+/F' pro+lac i+z+y+)
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*Strain: A-3245 (pro-lac i-z+y+F' pro+lac i-z+y+)
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*Strain: ML308 (lac i-z+y+)
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*Strain: ML308-225 (lac i-z-y+)
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*Thiodi[3H]galactoside (TDG) binding assays
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*Cell-free fractions/ membrane particles
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{|  id="K5058e3e1195b3"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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==Notes==
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==References==
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<references/>
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[[Category:Publication]]

Latest revision as of 16:14, 18 September 2012

Citation

Kennedy, EP , Rumley, MK and Armstrong, JB (1974) Dierect measurement of the binding of labeled sugars to the lactose permease M protein. J. Biol. Chem. 249:33-7

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Bacterial Proteins/metabolism; Cell Membrane/drug effects; Cell Membrane/enzymology; Cell-Free System; Chromatography, Ion Exchange; Escherichia coli/cytology; Escherichia coli/enzymology; Galactose; Hydrogen-Ion Concentration; Isotope Labeling; Kinetics; Lactose; Membrane Transport Modulators; Membrane Transport Proteins/antagonists & inhibitors; Membrane Transport Proteins/metabolism; Methods; Mutation; Phosphorus Radioisotopes; Receptors, Drug; Structure-Activity Relationship; Surface-Active Agents/pharmacology; Thioglycosides/metabolism; Time Factors; Tritium

Main Points of the Paper

Please summarize the main points of the paper.

  • Describes a method for measuring the binding of labeled sugars to M (LacY) directly

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).


  • Strain: A-324 (pro-lac i-z+y+F-)
  • Strain: A-3244 (pro-lac i-z+y+/F' pro+lac i+z+y+)
  • Strain: A-3245 (pro-lac i-z+y+F' pro+lac i-z+y+)
  • Strain: ML308 (lac i-z+y+)
  • Strain: ML308-225 (lac i-z-y+)
  • Thiodi[3H]galactoside (TDG) binding assays
  • Cell-free fractions/ membrane particles

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

</protect>

Notes

References

See Help:References for how to manage references in omp dev.