Difference between revisions of "PMID:4588694"
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+ | {{RightTOC}} | ||
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+ | * | ||
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+ | {| id="X5058e3e108452" class=" tableEdit PMID_info_table" | ||
+ | |||
+ | |- | ||
+ | !align=left |Citation | ||
+ | || | ||
+ | '''Kennedy, EP , Rumley, MK and Armstrong, JB ''' (1974) Dierect measurement of the binding of labeled sugars to the lactose permease M protein. ''J. Biol. Chem.'' '''249''':33-7 | ||
+ | |- | ||
+ | !align=left |Abstract | ||
+ | || | ||
+ | No abstract in PubMed | ||
+ | |- | ||
+ | !align=left |Links | ||
+ | || | ||
+ | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4588694 PubMed] | ||
+ | |||
+ | |- | ||
+ | !align=left |Keywords | ||
+ | || | ||
+ | Bacterial Proteins/metabolism; Cell Membrane/drug effects; Cell Membrane/enzymology; Cell-Free System; Chromatography, Ion Exchange; Escherichia coli/cytology; Escherichia coli/enzymology; Galactose; Hydrogen-Ion Concentration; Isotope Labeling; Kinetics; Lactose; Membrane Transport Modulators; Membrane Transport Proteins/antagonists & inhibitors; Membrane Transport Proteins/metabolism; Methods; Mutation; Phosphorus Radioisotopes; Receptors, Drug; Structure-Activity Relationship; Surface-Active Agents/pharmacology; Thioglycosides/metabolism; Time Factors; Tritium | ||
+ | |||
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+ | |} | ||
+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3507.X5058e3e108452--> | ||
+ | |||
+ | ==Main Points of the Paper == | ||
+ | {{LitSignificance}} | ||
+ | |||
+ | *Describes a method for measuring the binding of labeled sugars to M (LacY) directly | ||
+ | |||
+ | == Materials and Methods Used == | ||
+ | {{LitMaterials}} | ||
+ | |||
+ | |||
+ | *Strain: A-324 (pro-lac i-z+y+F-) | ||
+ | *Strain: A-3244 (pro-lac i-z+y+/F' pro+lac i+z+y+) | ||
+ | *Strain: A-3245 (pro-lac i-z+y+F' pro+lac i-z+y+) | ||
+ | *Strain: ML308 (lac i-z+y+) | ||
+ | *Strain: ML308-225 (lac i-z-y+) | ||
+ | *Thiodi[3H]galactoside (TDG) binding assays | ||
+ | *Cell-free fractions/ membrane particles | ||
+ | |||
+ | ==Phenotype Annotations== | ||
+ | {{AnnotationTableHelp}} | ||
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+ | <!-- | ||
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+ | * | ||
+ | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
+ | * | ||
+ | ****************************************************************************************** --> | ||
+ | {| id="K5058e3e1195b3" class=" tableEdit Phenotype_Table_2" | ||
+ | |- | ||
+ | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
+ | |||
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+ | |} | ||
+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3507.K5058e3e1195b3--></protect> | ||
+ | |||
+ | ==Notes== | ||
+ | |||
+ | ==References== | ||
+ | {{RefHelp}} | ||
+ | <references/> | ||
+ | |||
+ | |||
+ | [[Category:Publication]] |
Latest revision as of 16:14, 18 September 2012
Citation |
Kennedy, EP , Rumley, MK and Armstrong, JB (1974) Dierect measurement of the binding of labeled sugars to the lactose permease M protein. J. Biol. Chem. 249:33-7 |
---|---|
Abstract |
No abstract in PubMed |
Links | |
Keywords |
Bacterial Proteins/metabolism; Cell Membrane/drug effects; Cell Membrane/enzymology; Cell-Free System; Chromatography, Ion Exchange; Escherichia coli/cytology; Escherichia coli/enzymology; Galactose; Hydrogen-Ion Concentration; Isotope Labeling; Kinetics; Lactose; Membrane Transport Modulators; Membrane Transport Proteins/antagonists & inhibitors; Membrane Transport Proteins/metabolism; Methods; Mutation; Phosphorus Radioisotopes; Receptors, Drug; Structure-Activity Relationship; Surface-Active Agents/pharmacology; Thioglycosides/metabolism; Time Factors; Tritium |
edit table |
Main Points of the Paper
Please summarize the main points of the paper.
- Describes a method for measuring the binding of labeled sugars to M (LacY) directly
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- Strain: A-324 (pro-lac i-z+y+F-)
- Strain: A-3244 (pro-lac i-z+y+/F' pro+lac i+z+y+)
- Strain: A-3245 (pro-lac i-z+y+F' pro+lac i-z+y+)
- Strain: ML308 (lac i-z+y+)
- Strain: ML308-225 (lac i-z-y+)
- Thiodi[3H]galactoside (TDG) binding assays
- Cell-free fractions/ membrane particles
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
---|---|---|---|---|---|---|---|---|---|
edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.