Difference between revisions of "PMID:375003"

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{|  id="F509459b4640a5"  class=" tableEdit PMID_info_table" 
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'''Verhoef, C , Lugtenberg, B , van Boxtel, R , de Graaff, P  and Verheij, H '''  (1979) Genetics and biochemistry of the peptidoglycan-associated proteins b and c of Escherichia coli K12. ''Mol. Gen. Genet.'' '''169''':137-46
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!align=left  |Abstract
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To collect information on synthesis and regulation of the peptidoglycan-associated pore-forming outer membrane proteins b and c, mutants resistant to phages Me1 and TuIa were analyzed. Genetic analysis showed three linkage groups, corresponding with the genes tolF (phenotype b-c+), meoA (phenotype b+c-) and ompB (phenotypes b-c-, b-c+, b++c- and b++c+/-). It has recently been described that also a b+c- phenotype can occur in the latter linkage group [Chai, T., Foulds, J., J. Bacteriol. 130, 781-786 (1977)]. Among ompB (b-c+)/meoA (b+c-) double mutants strains were found with the b+c- phenotype, showing that ompB is not the structural gene for protein b. Studies on purified proteins b and c showed profound differences between the two proteins with respect to the electrophoretic mobility of fragments obtained by treatment with cyanogen bromide, trypsin and chymotrypsin. The amino acid in position three of the amino-termini of proteins b and c, isolated from isogenic strains, were identified as isoleucine and valine respectively. Both the genetic and biochemical results are consistent with a model recently published [Ichihara, S., Mizushima, S., J. Biochem. (Japan) 83, 1095-1100 (1978)] which predicts that tolF and meoA are the structural genes for the proteins b and c respectively and that ompB is a regulatory gene whose product regulates the levels of both proteins.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=375003 PubMed]
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!align=left  |Keywords
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Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/genetics; Genes; Genes, Regulator; Genetic Linkage; Membrane Proteins/genetics; Membrane Proteins/isolation & purification; Mutation; Peptidoglycan/genetics; Phenotype
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{|  id="H509459b4b47f7"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: PC0479
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''envZ''
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*Genotype of Experimental Strain : ''envZ'' in strain CE1115
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*Reference Condition:
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Resistant to Me1 phage.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: PC0479
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ompC''
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*Genotype of Experimental Strain : ''ompC'' in strain CE104
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*Reference Condition:
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Resistant to Me1 phage.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: PC0479
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ompF''
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*Genotype of Experimental Strain : ''ompF'' in mutant strain CE1121
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*Reference Condition:
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Resistant to TuIa phage.
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]] [[Category:LaRossa citations that need review]]

Latest revision as of 15:39, 5 November 2012

Citation

Verhoef, C , Lugtenberg, B , van Boxtel, R , de Graaff, P and Verheij, H (1979) Genetics and biochemistry of the peptidoglycan-associated proteins b and c of Escherichia coli K12. Mol. Gen. Genet. 169:137-46

Abstract

To collect information on synthesis and regulation of the peptidoglycan-associated pore-forming outer membrane proteins b and c, mutants resistant to phages Me1 and TuIa were analyzed. Genetic analysis showed three linkage groups, corresponding with the genes tolF (phenotype b-c+), meoA (phenotype b+c-) and ompB (phenotypes b-c-, b-c+, b++c- and b++c+/-). It has recently been described that also a b+c- phenotype can occur in the latter linkage group [Chai, T., Foulds, J., J. Bacteriol. 130, 781-786 (1977)]. Among ompB (b-c+)/meoA (b+c-) double mutants strains were found with the b+c- phenotype, showing that ompB is not the structural gene for protein b. Studies on purified proteins b and c showed profound differences between the two proteins with respect to the electrophoretic mobility of fragments obtained by treatment with cyanogen bromide, trypsin and chymotrypsin. The amino acid in position three of the amino-termini of proteins b and c, isolated from isogenic strains, were identified as isoleucine and valine respectively. Both the genetic and biochemical results are consistent with a model recently published [Ichihara, S., Mizushima, S., J. Biochem. (Japan) 83, 1095-1100 (1978)] which predicts that tolF and meoA are the structural genes for the proteins b and c respectively and that ompB is a regulatory gene whose product regulates the levels of both proteins.

Links

PubMed

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/genetics; Genes; Genes, Regulator; Genetic Linkage; Membrane Proteins/genetics; Membrane Proteins/isolation & purification; Mutation; Peptidoglycan/genetics; Phenotype

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: PC0479
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: envZ
  • Genotype of Experimental Strain : envZ in strain CE1115
  • Reference Condition:

Resistant to Me1 phage.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: PC0479
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ompC
  • Genotype of Experimental Strain : ompC in strain CE104
  • Reference Condition:

Resistant to Me1 phage.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: PC0479
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ompF
  • Genotype of Experimental Strain : ompF in mutant strain CE1121
  • Reference Condition:

Resistant to TuIa phage.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:375003&oldid=8698"