Difference between revisions of "PMID:350837"

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{|  id="T509c03d881c09"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Duncan, BK , Rockstroh, PA  and Warner, HR '''  (1978) Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase. ''J. Bacteriol.'' '''134''':1039-45
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!align=left  |Abstract
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A new assay specific for uracil-DNA glycosylase is described, Escherichia coli mutants partially and totally deficient in uracil-DNA glycosylase activity have been isolated by using this assay in mass-screening procedures. These have been designated ung mutants. The ung gene maps between tyrA and nadB on the E. coli chromosome. T4 phage containing uracil in their DNA grow on the most glycosylase-deficient hosts but are unable to grow on wild-type bacteria. This provides a simple spot test for the ung genotype. The ung mutants show slightly higher rates of spontaneous mutation to antibiotic resistance. Taken together, these results suggest a central role for uracil-DNA glycosylase in the initiation of an excision repair pathway for the exclusion of uracil from DNA.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=350837 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC222353 PMC222353]
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!align=left  |Keywords
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Chromosome Mapping; Coliphages/analysis; Coliphages/growth & development; DNA Repair; DNA, Viral/analysis; Deoxyuracil Nucleotides; Escherichia coli/genetics; Genes; Mutation; N-Glycosyl Hydrolases/genetics; Uracil/analysis
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="P509c03d8c777c"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: W3110
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ung<sup>+</sup>''
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*Genotype of Experimental Strain : ''ung<sup>-</sup> in strain BD10''
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*Reference Condition: 25<sup>o</sup> C
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*Experimental Condition: 42<sup>o</sup>C
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0000298
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enzyme activity phenotype
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0000178
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in vivo assay evidence
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''ung'' mutation caused reduced Uracil-DNA glycosylase activity at 42 <sup>o</sup> C. Also seen in strain BD13.  See table 2.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: W3110
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ung<sup>+</sup>''
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*Genotype of Experimental Strain : ''ung-6<sup>-</sup>'' in strain BD1124''
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*Reference Condition: 25<sup>o</sup> C
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*Experimental Condition: 42<sup>o</sup>C
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0000298
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enzyme activity phenotype
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|
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0000178
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|
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in vivo assay evidence
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|
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''ung'' mutation caused reduced Uracil-DNA glycosylase activity at 45 <sup>o</sup> C, roughly 95 percent compared to the wild type.  See figure 1 for full results. 
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|-
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: W3110
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ung<sup>+</sup>''
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*Genotype of Experimental Strain : ''ung-6<sup>-</sup>'' in strain BD1124''
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|
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*Reference Condition: 25<sup>o</sup> C
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*Experimental Condition: 42<sup>o</sup>C
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|
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0000298
 +
|
 +
enzyme activity phenotype
 +
|
 +
0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
''ung'' mutation caused reduced Uracil-DNA glycosylase activity at 45 <sup>o</sup> C, roughly 95 percent compared to the wild type.  See figure 1 for full results. 
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|
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]]
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[[Category:Papers referenced in the LaRossa chapter]]

Latest revision as of 13:42, 14 December 2012

Citation

Duncan, BK , Rockstroh, PA and Warner, HR (1978) Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase. J. Bacteriol. 134:1039-45

Abstract

A new assay specific for uracil-DNA glycosylase is described, Escherichia coli mutants partially and totally deficient in uracil-DNA glycosylase activity have been isolated by using this assay in mass-screening procedures. These have been designated ung mutants. The ung gene maps between tyrA and nadB on the E. coli chromosome. T4 phage containing uracil in their DNA grow on the most glycosylase-deficient hosts but are unable to grow on wild-type bacteria. This provides a simple spot test for the ung genotype. The ung mutants show slightly higher rates of spontaneous mutation to antibiotic resistance. Taken together, these results suggest a central role for uracil-DNA glycosylase in the initiation of an excision repair pathway for the exclusion of uracil from DNA.

Links

PubMed PMC222353

Keywords

Chromosome Mapping; Coliphages/analysis; Coliphages/growth & development; DNA Repair; DNA, Viral/analysis; Deoxyuracil Nucleotides; Escherichia coli/genetics; Genes; Mutation; N-Glycosyl Hydrolases/genetics; Uracil/analysis

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: W3110
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ung+
  • Genotype of Experimental Strain : ung- in strain BD10
  • Reference Condition: 25o C
  • Experimental Condition: 42oC

0000298

enzyme activity phenotype

0000178

in vivo assay evidence

ung mutation caused reduced Uracil-DNA glycosylase activity at 42 o C. Also seen in strain BD13. See table 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: W3110
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ung+
  • Genotype of Experimental Strain : ung-6- in strain BD1124
  • Reference Condition: 25o C
  • Experimental Condition: 42oC

0000298

enzyme activity phenotype

0000178

in vivo assay evidence

ung mutation caused reduced Uracil-DNA glycosylase activity at 45 o C, roughly 95 percent compared to the wild type. See figure 1 for full results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: W3110
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ung+
  • Genotype of Experimental Strain : ung-6- in strain BD1124
  • Reference Condition: 25o C
  • Experimental Condition: 42oC

0000298

enzyme activity phenotype

0000178

in vivo assay evidence

ung mutation caused reduced Uracil-DNA glycosylase activity at 45 o C, roughly 95 percent compared to the wild type. See figure 1 for full results.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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