Difference between revisions of "PMID:3546268"

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'''Abdulrashid, N  and Clark, DP '''  (1987) Isolation and genetic analysis of mutations allowing the degradation of furans and thiophenes by Escherichia coli. ''J. Bacteriol.'' '''169''':1267-71
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Successive mutations of Escherichia coli yielded a strain that was able to degrade a variety of heterocyclic oxygen- and sulfur-containing ring compounds. In particular, this strain could use both furan-2-carboxylic acid and thiophene-2-carboxylic acid as sole carbon and energy sources. Nitrogen-containing heterocyclic compounds were not degraded. This mutant was isolated by selecting first for oxidation of furan derivatives and then for thiophene degradation. Genetic analysis revealed that mutations in three novel genes, thdA (12 min), thdC (92 min), and thdD (98 min), were required for thiophene degradation. In addition, constitutively at both of the previously characterized fadR and atoC loci was required for efficient thiophene breakdown. The pathway of furan and thiophene degradation remains obscure, but the inability of our mutants to degrade 5-nitro- or 5-bromo-substituted furan derivatives suggests that hydroxylation at position 5 may be involved. Thiophene derivatives were toxic when they were present at concentrations of 0.1% or greater; however, addition of trace amounts of phenylalanine plus tyrosine greatly reduced this effect.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3546268 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211929 PMC211929]
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Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Furans/metabolism; Mutation; Oxidation-Reduction; Species Specificity; Structure-Activity Relationship; Thiophenes/metabolism
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 08:03, 12 December 2012

Citation

Abdulrashid, N and Clark, DP (1987) Isolation and genetic analysis of mutations allowing the degradation of furans and thiophenes by Escherichia coli. J. Bacteriol. 169:1267-71

Abstract

Successive mutations of Escherichia coli yielded a strain that was able to degrade a variety of heterocyclic oxygen- and sulfur-containing ring compounds. In particular, this strain could use both furan-2-carboxylic acid and thiophene-2-carboxylic acid as sole carbon and energy sources. Nitrogen-containing heterocyclic compounds were not degraded. This mutant was isolated by selecting first for oxidation of furan derivatives and then for thiophene degradation. Genetic analysis revealed that mutations in three novel genes, thdA (12 min), thdC (92 min), and thdD (98 min), were required for thiophene degradation. In addition, constitutively at both of the previously characterized fadR and atoC loci was required for efficient thiophene breakdown. The pathway of furan and thiophene degradation remains obscure, but the inability of our mutants to degrade 5-nitro- or 5-bromo-substituted furan derivatives suggests that hydroxylation at position 5 may be involved. Thiophene derivatives were toxic when they were present at concentrations of 0.1% or greater; however, addition of trace amounts of phenylalanine plus tyrosine greatly reduced this effect.

Links

PubMed PMC211929

Keywords

Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Furans/metabolism; Mutation; Oxidation-Reduction; Species Specificity; Structure-Activity Relationship; Thiophenes/metabolism

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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