Difference between revisions of "PMID:6337124"
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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*Reference Condition: | *Reference Condition: | ||
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| − | OMP: | + | OMP:0006058 |
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| − | increased resistance to | + | increased resistance to aminoglycoside |
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| − | 0000033 | + | ECO:0000033 |
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traceable author statement | traceable author statement | ||
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*Reference Condition: | *Reference Condition: | ||
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| − | OMP: | + | OMP:0006058 |
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| − | increased resistance to | + | increased resistance to aminoglycoside |
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| − | 0000033 | + | ECO:0000033 |
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traceable author statement | traceable author statement | ||
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*Reference Condition: | *Reference Condition: | ||
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| − | OMP: | + | OMP:0006058 |
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| − | increased resistance to | + | increased resistance to aminoglycoside |
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| − | 0000181 | + | ECO:0000181 |
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in vitro assay data | in vitro assay data | ||
Latest revision as of 16:48, 3 August 2015
| Citation |
Dabbs, ER (1983) Escherichia coli kasugamycin dependence arising from mutation at the rpsI locus.J. Bacteriol. 153:709-15 |
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| Abstract |
Escherichia coli mutants with alterations in the electrophoretic mobility of ribosomal protein S9 were used to locate rpsI, the gene for this protein, on the linkage map. rpsI was located at about 70 min, roughly halfway between argG and fabE. It was very close to the gene for ribosomal protein L13, rplM. Another mutation at the rpsI locus gave rise to a phenotype of kasugamycin dependence and resistance. In this mutant, dependence on antibiotic came from kasugamycin being necessary to slow the rate of protein synthesis. |
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| Keywords |
Aminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli; Genes, Bacterial; Mutation; Phenotype; Ribosomal Proteins |
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Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
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OMP:0006058 |
increased resistance to aminoglycoside |
ECO:0000033 |
traceable author statement |
Due to alterations in the rpsI gene there was an increase in kasugamycin resistance. See page 712 of for full details and Fig 1 for two-dimensional gel electropherogram of MV101 strain. |
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a mutation or genetic difference within a strain |
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|
|
OMP:0006058 |
increased resistance to aminoglycoside |
ECO:0000033 |
traceable author statement |
Due to alterations in the rpsI gene kasugamycin dependence developed. See page 712 of for full details and Fig 1 for two-dimensional gel electropherogram of MV101 strain. |
|
|
a mutation or genetic difference within a strain |
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|
|
OMP:0006058 |
increased resistance to aminoglycoside |
ECO:0000181 |
in vitro assay data |
Kasugamycin dependence due to rpsI mutation was verified by antibiotic deprivation. See Figure 3 for growth curve of antibiotic test. See figure 4 for rate of protein and nucleic acid synthesis. |
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</protect>
Notes
References
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