Difference between revisions of "PMID:8631969"

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{|  id="L513634b428889"  class=" tableEdit PMID_info_table" 
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'''Brooke, JS and Valvano, MA'''  (1996) Biosynthesis of inner core lipopolysaccharide in enteric bacteria identification and characterization of a conserved phosphoheptose isomerase. ''J. Biol. Chem.'' '''271''':3608-14
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!align=left  |Abstract
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The lpcA locus has been identified in Escherichia coli K12 novobiocin-supersensitive mutants that produce a short lipopolysaccharide (LPS) core which lacks glyceromannoheptose and terminal hexoses. We have characterized lpcA as a single gene mapping around 5.3 min (246 kilobases) on the E. coli K12 chromosome and encoding a 22.6-kDa cytosolic protein. Recombinant plasmids containing only lpcA restored a complete core LPS in the E. coli strain chi711. We show that this strain has an IS5-mediated chromosomal deletion of 35 kilobases that eliminates lpcA. The LpcA protein showed discrete similarities with a family of aldose/ketose isomerases and other proteins of unknown function. The isomerization of sedoheptulose 7-phosphate, into a phosphosugar presumed to be D-glycero-D-mannoheptose 7-phosphate, was detected in enzyme reactions with cell extracts of E. coli lpcA+ and of lpcA mutants containing the recombinant lpcA gene. We concluded that LpcA is the phosphoheptose isomerase used in the first step of glyceromannoheptose synthesis. We also demonstrated that lpcA is conserved among enteric bacteria, all of which contain glyceromannoheptose in the inner core LPS, indicating that LpcA is an essential component in a conserved biosynthetic pathway of inner core LPS.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8631969 PubMed]
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!align=left  |Keywords
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Aldose-Ketose Isomerases; Amino Acid Sequence; Animals; Base Sequence; Carbohydrate Epimerases/chemistry; Carbohydrate Epimerases/genetics; Carbohydrate Epimerases/metabolism; Carbohydrate Metabolism; Carbohydrates/analysis; Chromatography, High Pressure Liquid; Chromosome Deletion; Chromosomes, Bacterial; Conserved Sequence; Enterobacteriaceae/enzymology; Enterobacteriaceae/genetics; Enterobacteriaceae/immunology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/immunology; Genes, Bacterial; Genotype; Lipopolysaccharides/biosynthesis; Molecular Sequence Data; Rats; Restriction Mapping; Sequence Homology, Amino Acid
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="J513634b478760"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: ''<sub>X</sub>''705
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''lpcA<sup>+</sup>''
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*Genotype of Experimental Strain : ''lpcA<sup>-</sup>'' in Strain ''<sub>X</sub>''711
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*Reference Condition:
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The mutation caused a decrease in the size of the core oligosaccharide.  See figure 1 lane 2. 
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: ''<sub>X</sub>''705
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''rfa<sup>+</sup>''
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*Genotype of Experimental Strain : rfa-1 in D21e7
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*Reference Condition:
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The mutation caused a the production of a shorter LPS core though not as small as ''<sub>X</sub>''711.  See figure 1 lane 5. 
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: ''<sub>X</sub>''705
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''rfa<sup>+</sup>''
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*Genotype of Experimental Strain : ''rfaG(del) rfaP(del) rfaM(del) rfaN(del) rfaB(del)''::CS2052
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*Reference Condition:
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The mutation caused a the production of a shorter LPS core though not as small as ''<sub>X</sub>''711.  See figure 1 lane 6. 
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==Notes==
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*Based on LPS analysis it was concluded that ''<sub>X</sub>''711 lacks heptose in its core LPS.
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]]

Latest revision as of 16:07, 5 March 2013

Citation

Brooke, JS and Valvano, MA (1996) Biosynthesis of inner core lipopolysaccharide in enteric bacteria identification and characterization of a conserved phosphoheptose isomerase. J. Biol. Chem. 271:3608-14

Abstract

The lpcA locus has been identified in Escherichia coli K12 novobiocin-supersensitive mutants that produce a short lipopolysaccharide (LPS) core which lacks glyceromannoheptose and terminal hexoses. We have characterized lpcA as a single gene mapping around 5.3 min (246 kilobases) on the E. coli K12 chromosome and encoding a 22.6-kDa cytosolic protein. Recombinant plasmids containing only lpcA restored a complete core LPS in the E. coli strain chi711. We show that this strain has an IS5-mediated chromosomal deletion of 35 kilobases that eliminates lpcA. The LpcA protein showed discrete similarities with a family of aldose/ketose isomerases and other proteins of unknown function. The isomerization of sedoheptulose 7-phosphate, into a phosphosugar presumed to be D-glycero-D-mannoheptose 7-phosphate, was detected in enzyme reactions with cell extracts of E. coli lpcA+ and of lpcA mutants containing the recombinant lpcA gene. We concluded that LpcA is the phosphoheptose isomerase used in the first step of glyceromannoheptose synthesis. We also demonstrated that lpcA is conserved among enteric bacteria, all of which contain glyceromannoheptose in the inner core LPS, indicating that LpcA is an essential component in a conserved biosynthetic pathway of inner core LPS.

Links

PubMed

Keywords

Aldose-Ketose Isomerases; Amino Acid Sequence; Animals; Base Sequence; Carbohydrate Epimerases/chemistry; Carbohydrate Epimerases/genetics; Carbohydrate Epimerases/metabolism; Carbohydrate Metabolism; Carbohydrates/analysis; Chromatography, High Pressure Liquid; Chromosome Deletion; Chromosomes, Bacterial; Conserved Sequence; Enterobacteriaceae/enzymology; Enterobacteriaceae/genetics; Enterobacteriaceae/immunology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/immunology; Genes, Bacterial; Genotype; Lipopolysaccharides/biosynthesis; Molecular Sequence Data; Rats; Restriction Mapping; Sequence Homology, Amino Acid

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: X705
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: lpcA+
  • Genotype of Experimental Strain : lpcA- in Strain X711
  • Reference Condition:

The mutation caused a decrease in the size of the core oligosaccharide. See figure 1 lane 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: X705
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rfa+
  • Genotype of Experimental Strain : rfa-1 in D21e7
  • Reference Condition:

The mutation caused a the production of a shorter LPS core though not as small as X711. See figure 1 lane 5.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: X705
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rfa+
  • Genotype of Experimental Strain : rfaG(del) rfaP(del) rfaM(del) rfaN(del) rfaB(del)::CS2052
  • Reference Condition:

The mutation caused a the production of a shorter LPS core though not as small as X711. See figure 1 lane 6.


edit table

</protect>

Notes

  • Based on LPS analysis it was concluded that X711 lacks heptose in its core LPS.

References

See Help:References for how to manage references in omp dev.

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