PMID:14153532

Citation	WITKIN, EM and SICURELLA, NA (1964) PURE CLONES OF LACTOSE-NEGATIVE MUTANTS OBTAINED IN ESCHERICHIA COLI AFTER TREATMENT WITH 5-BROMOURACIL. J. Mol. Biol. 8:610-3
Abstract	No abstract in PubMed
Links	PubMed
Keywords
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Main Points of the Paper

Please summarize the main points of the paper.

• Describes the introduction of mutations (by incorporation of 5BU into DNA) that results in cells unable to ferment lactose
• Sectoring phenotypes appear, indicative of the timing the mutation gets incorporated within a cell of a colony

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

• Parent strain: Z-15 (radiation-resistant, serine-requiring variant of the thymineless strain B3)

• 5-Bromouracil Mutagenesis (on plates)
  • strain plated on 5BU completed 1 round of replication, whereby most of the thymine was replaced by 5BU
  • strain plated on thymine (control) completed a normal round of DNA replication
• Bacto-eosin-methylene blue (EMB) indicator agar (for more information, click here)
  • dark red colonies (lactose +)
  • colorless colonies (lactose -)
  • mixed
    • large sectors (lac- component originated at the center and constituted one-eighth or more the area of the colony)
    • peripheral sectors

Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: z-15 NCBI Taxon ID: 83333	Genotype of Reference Strain: Strain z-15 Genotype of Experimental Strain : Derivatives of Z-15	Reference Condition:	OMP:0000284	loss of utilization of carbon-containing compound			Abolished utilization of carbon source lactose in mutant strain. See Table 1.
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References

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