Difference between revisions of "PMID:1989883"

From omp dev
Jump to: navigation, search
(New PMID: Page!)
 
(Fill PMID: Page!)
Line 1: Line 1:
 +
{{RightTOC}}
  
 +
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.309.O4d5c3a5540752-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="O4d5c3a5540752"  class=" tableEdit PMID_info_table" 
 +
 +
|-
 +
!align=left  |Citation
 +
||
 +
'''Niki, H, Jaffé, A, Imamura, R, Ogura, T and Hiraga, S'''  (1991) The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli.''EMBO J.'' '''10''':183-93
 +
|-
 +
!align=left  |Abstract
 +
||
 +
An Escherichia coli temperature sensitive mutant which produces spontaneously normal size anucleate cells at low temperature was isolated. The mutant is defective in a previously undescribed gene, named mukB, located at 21 min on the chromosome. The mukB gene codes for a large protein (approximately 180 kd). A 1534 amino acid protein (176,826 daltons) was deduced from the nucleotide sequence of the mukB gene. Computer analysis revealed that the predicted MukB protein has distinct domains: an amino-terminal globular domain containing a nucleotide binding sequence, a central region containing two alpha-helical coiled-coil domains and one globular domain, and a carboxyl-terminal globular domain which is rich in Cys, Arg and Lys. A 180 kd protein detected in wild-type cell extracts by electrophoresis is absent in mukB null mutants. Although the null mutants are not lethal at low temperature, the absence of MukB leads to aberrant chromosome partitioning. At high temperature the mukB null mutants cannot form colonies and many nucleoids are distributed irregularly along elongated cells. We conclude that the MukB protein is required for chromosome partitioning in E. coli.
 +
|-
 +
!align=left  |Links
 +
||
 +
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1989883 PubMed]
 +
 +
|-
 +
!align=left  |Keywords
 +
||
 +
Amino Acid Sequence; Bacterial Proteins; Base Sequence; Chromosomal Proteins, Non-Histone; Chromosomes, Bacterial; Cloning, Molecular; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; Genotype; Molecular Sequence Data; Molecular Weight; Mutation; Protein Conformation; Restriction Mapping; Sequence Homology, Nucleic Acid
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.309.O4d5c3a5540752&page=309&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 +
|}
 +
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.309.O4d5c3a5540752-->
 +
 +
==Main Points of the Paper ==
 +
{{LitSignificance}}
 +
 +
== Materials and Methods Used ==
 +
{{LitMaterials}}
 +
 +
==Phenotype Annotations==
 +
{{AnnotationTableHelp}}
 +
<protect><!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.309.H4d5c3a555bf84-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="H4d5c3a555bf84"  class=" tableEdit PMID_Phenotype_table" 
 +
|-
 +
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.309.H4d5c3a555bf84&page=309&pagename={{FULLPAGENAMEE}}&type=0&template=PMID_Phenotype_table edit table]</span> || || || || || || || ||
 +
|}
 +
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.309.H4d5c3a555bf84--></protect>
 +
 +
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 +
<references/>
 +
 +
[[Category:Template]]

Revision as of 15:57, 16 February 2011

Citation

Niki, H, Jaffé, A, Imamura, R, Ogura, T and Hiraga, S (1991) The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli.EMBO J. 10:183-93

Abstract

An Escherichia coli temperature sensitive mutant which produces spontaneously normal size anucleate cells at low temperature was isolated. The mutant is defective in a previously undescribed gene, named mukB, located at 21 min on the chromosome. The mukB gene codes for a large protein (approximately 180 kd). A 1534 amino acid protein (176,826 daltons) was deduced from the nucleotide sequence of the mukB gene. Computer analysis revealed that the predicted MukB protein has distinct domains: an amino-terminal globular domain containing a nucleotide binding sequence, a central region containing two alpha-helical coiled-coil domains and one globular domain, and a carboxyl-terminal globular domain which is rich in Cys, Arg and Lys. A 180 kd protein detected in wild-type cell extracts by electrophoresis is absent in mukB null mutants. Although the null mutants are not lethal at low temperature, the absence of MukB leads to aberrant chromosome partitioning. At high temperature the mukB null mutants cannot form colonies and many nucleoids are distributed irregularly along elongated cells. We conclude that the MukB protein is required for chromosome partitioning in E. coli.

Links

PubMed

Keywords

Amino Acid Sequence; Bacterial Proteins; Base Sequence; Chromosomal Proteins, Non-Histone; Chromosomes, Bacterial; Cloning, Molecular; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; Genotype; Molecular Sequence Data; Molecular Weight; Mutation; Protein Conformation; Restriction Mapping; Sequence Homology, Nucleic Acid

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes
edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:1989883&oldid=1296"