PMID:1989883

Citation	Niki, H, Jaffé, A, Imamura, R, Ogura, T and Hiraga, S (1991) The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli.EMBO J. 10:183-93
Abstract	An Escherichia coli temperature sensitive mutant which produces spontaneously normal size anucleate cells at low temperature was isolated. The mutant is defective in a previously undescribed gene, named mukB, located at 21 min on the chromosome. The mukB gene codes for a large protein (approximately 180 kd). A 1534 amino acid protein (176,826 daltons) was deduced from the nucleotide sequence of the mukB gene. Computer analysis revealed that the predicted MukB protein has distinct domains: an amino-terminal globular domain containing a nucleotide binding sequence, a central region containing two alpha-helical coiled-coil domains and one globular domain, and a carboxyl-terminal globular domain which is rich in Cys, Arg and Lys. A 180 kd protein detected in wild-type cell extracts by electrophoresis is absent in mukB null mutants. Although the null mutants are not lethal at low temperature, the absence of MukB leads to aberrant chromosome partitioning. At high temperature the mukB null mutants cannot form colonies and many nucleoids are distributed irregularly along elongated cells. We conclude that the MukB protein is required for chromosome partitioning in E. coli.
Links	PubMed
Keywords	Amino Acid Sequence; Bacterial Proteins; Base Sequence; Chromosomal Proteins, Non-Histone; Chromosomes, Bacterial; Cloning, Molecular; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; Genotype; Molecular Sequence Data; Molecular Weight; Mutation; Protein Conformation; Restriction Mapping; Sequence Homology, Nucleic Acid
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Main Points of the Paper

• Describes the identification of mukB
• MukB is involved in chromosome partitioning

Materials and Methods Used

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Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli W3110	NCBI:316407	SH3367	mukB106	Aberrant chromosome partitioning at low temperature (conditional)	Morphology	nucleoid morphology of cell	Microscopy	Figure 1
Escherichia coli W3110	NCBI:316407	SH3367	mukB106	Increased number of anucleate cells at low temperature	Morphology	nucleoid morphology of cell	Microscopy	Figure 1
Escherichia coli W3110	NCBI:316407	SH3367	mukB106	Increased production of anucleate cells during growth	Morphology	nucleoid morphology of cell	Microscopy	Table 1
Escherichia coli W3110	NCBI:316407	SH3367	ΔmukB	Abolished colony forming ability at high temperature	Growth	plating at high temperature	Plating Assay	Figure 4
Escherichia coli W3110	NCBI:316407	SH3367	mukB106	Increased number of anucleate cells at low temperature	Morphology	nucleoid morphology of cell	Microscopy	Figure 1
Escherichia coli W3110	NCBI:316407	SH3367	mukB106	Partially defective in cell division at high temperature.	Morphology	morphology of cell	Microscopy	Figure 1
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References

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