Difference between revisions of "PMID:1931824"
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==Main Points of the Paper == | ==Main Points of the Paper == | ||
| − | + | *''xerC'' insertion mutants have defects in cell division and chromosomal nucleoid segregation | |
| + | *site-specific recombination locus ''dif'' is related to ''cer'' | ||
| + | *''dif'' is a substrate for XerC-mediated site-specific recombination | ||
== Materials and Methods Used == | == Materials and Methods Used == | ||
Revision as of 15:07, 8 March 2011
| Citation |
Blakely, G, Colloms, S, May, G, Burke, M and Sherratt, D (1991) Escherichia coli XerC recombinase is required for chromosomal segregation at cell division.New Biol. 3:789-98 |
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| Abstract |
XerC is a site-specific recombinase of the bacteriophage lambda integrase family that is encoded by xerC at 3700 kbp on the genetic map of Escherichia coli. The protein was originally identified through its role in converting multimers of plasmid ColE1 to monomers; only monomers are stably inherited. Here we demonstrate that XerC also has a role in the segregation of replicated chromosomes at cell division. xerC mutants form filaments with aberrant nucleotides that appear unable to partition correctly. A DNA segment (dif) from the replication terminus region of the E. coli chromosome binds XerC and acts as a substrate for XerC-mediated site-specific recombination when inserted into multicopy plasmids. This dif segment contains a region of 28 bp with sequence similarity to the crossover region of ColE1 cer. The cell division phenotype of xerC mutants is suppressed in strains deficient in homologous recombination, suggesting that the role of XerC/dif in chromosomal metabolism is to convert any chromosomal multimers (arising through homologous recombination) to monomers. |
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| Keywords |
Aminopeptidases; Arginine; Bacteriocin Plasmids; Base Sequence; Binding Sites; Cell Division; Chromosome Mapping; Chromosomes, Bacterial; DNA Nucleotidyltransferases; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Glutamyl Aminopeptidase; Integrases; Molecular Sequence Data; Mutagenesis, Site-Directed; Phenotype; Recombinases; Recombination, Genetic; Repressor Proteins |
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Main Points of the Paper
- xerC insertion mutants have defects in cell division and chromosomal nucleoid segregation
- site-specific recombination locus dif is related to cer
- dif is a substrate for XerC-mediated site-specific recombination
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
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Notes
References
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