Difference between revisions of "PMID:20727918"

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(Table edited by Tkveton via TableEdit)
(Table edited by Tkveton via TableEdit)
Line 63: Line 63:
 
*Genotype of Experimental Strain : ''SOS-R1 pNfsA uvrB(del)''
 
*Genotype of Experimental Strain : ''SOS-R1 pNfsA uvrB(del)''
 
|
 
|
−
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micro molar CB1954
+
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 
|
 
|
−
OMP:0000172
+
OMP:0005023
 
|
 
|
−
response to chemical stimulus phenotype
+
presence of an SOS response
 
|
 
|
 
ECO:0000297
 
ECO:0000297
Line 73: Line 73:
 
LacZ transcript localization evidence
 
LacZ transcript localization evidence
 
|
 
|
−
Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromatest sensitivity;  
+
Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromotest response;  
 
Reference figure 2;
 
Reference figure 2;
 
NfsA (Ec)= 2-NO<sub>2</sub> nitroreductase only, from E. coli
 
NfsA (Ec)= 2-NO<sub>2</sub> nitroreductase only, from E. coli
  
−
Gene deletion enhances SOS chromatest assay sensitivity for nitroreductases which can be used for CB1954-mediated gene therapy.
 
 
|
 
|
 
   
 
   
Line 86: Line 85:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain:  
+
*Substrain: SOS-R1
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''uvrB''
+
*Genotype of Reference Strain: ''SOS-R1 pNfsA uvrB<sup>+</sup>''
−
*Genotype of Experimental Strain : ''uvrB(del)''
+
*Genotype of Experimental Strain : ''SOS-R1 pNfsA uvrB(del)''
 
|
 
|
−
*Reference Condition: CB1954 metabolism by NfsA (Kp)
+
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 
|
 
|
−
OMP:0000172
+
OMP:0005023
 
|
 
|
−
response to chemical stimulus phenotype
+
presence of an SOS response
 
|
 
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromotest response;
 +
Reference figure 2;
 +
NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pNfsB uvrB<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pNfsB uvrB(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromotest response;
 +
Reference figure 2;
 +
NfsB= 2-NO2 or 4-NO2 nitroreductase.
  
 
|
 
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pNemA uvrB<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pNemA uvrB(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005020
 +
|
 +
increased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2;
 +
NemA= 4-NO2 nitroreductase only.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pYfkO uvrB<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pYfkO uvrB(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005020
 +
|
 +
increased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2;
 +
Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pNfsA mutS<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pNfsA mutS(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response;
 +
Reference figure 2;
 +
NfsA (Ec)= 2-NO<sub>2</sub> nitroreductase only, from E. coli
  
 
|
 
|
−
Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromatest sensitivity;  
+
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pNfsA mutS<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pNfsA mutS(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response;
 +
Reference figure 2;
 +
NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pNfsB mutS<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pNfsB mutS(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response;
 +
Reference figure 2;
 +
NfsB= 2-NO2 or 4-NO2 nitroreductase.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pNemA mutS<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pNemA mutS(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response;
 +
Reference figure 2;
 +
NemA= 4-NO2 nitroreductase only.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pYfkO mutS<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pYfkO mutS(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response;
 +
Reference figure 2;
 +
Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pNfsA ada<sup>+</sup> ogt<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pNfsA ada(del) ogt(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response;  
 
Reference figure 2;
 
Reference figure 2;
−
NfsA (Kp)= 2-NO<sub>2</sub> nitroreductase only, from K. pneumoniae
+
NfsA (Ec)= 2-NO<sub>2</sub> nitroreductase only, from E. coli
  
 
|
 
|
Line 114: Line 356:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain:  
+
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 pNfsA ada<sup>+</sup> ogt<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SOS-R1 pNfsA ada(del) ogt(del)''
 +
|
 +
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response;
 +
Reference figure 2;
 +
NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''uvrB''
+
*Genotype of Reference Strain: ''SOS-R1 pNfsB ada<sup>+</sup> ogt<sup>+</sup>''
−
*Genotype of Experimental Strain : ''uvrB(del)''
+
*Genotype of Experimental Strain : ''SOS-R1 pNfsB ada(del) ogt(del)''
 
|
 
|
−
*Reference Condition: CB1954 metabolism by NfsB
+
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 
|
 
|
−
OMP:0000172
+
OMP:0005023
 
|
 
|
−
response to chemical stimulus phenotype
+
presence of an SOS response
 
|
 
|
−
 
+
ECO:0000297
 
|
 
|
−
 
+
LacZ transcript localization evidence
 
|
 
|
−
Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromatest sensitivity;  
+
Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response;  
−
Reference figure 2;
+
Reference figure 2;  
−
NfsB= 2-NO<sub>2</sub> or 4-NO<sub>2</sub> nitroreductase.
+
NfsB= 2-NO2 or 4-NO2 nitroreductase.
 
|
 
|
 
   
 
   
Line 141: Line 410:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain:  
+
*Substrain: SOS-R1
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''uvrB''
+
*Genotype of Reference Strain: ''SOS-R1 pNemA ada<sup>+</sup> ogt<sup>+</sup>''
−
*Genotype of Experimental Strain : ''uvrB(del)''
+
*Genotype of Experimental Strain : ''SOS-R1 pNemA ada(del) ogt(del)''
 
|
 
|
−
*Reference Condition: CB1954 metabolism by NemA
+
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 
|
 
|
−
OMP:0000172
+
OMP:0005023
 
|
 
|
−
response to chemical stimulus phenotype
+
presence of an SOS response
 
|
 
|
−
 
+
ECO:0000297
 
|
 
|
−
 
+
LacZ transcript localization evidence
 
|
 
|
−
Deletion of the uvrB nucleotide excision repair gene heightened the SOS chromatest sensitivity;  
+
Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response;
−
Reference figure 2;
+
Reference figure 2;  
−
NemA= 4-NO<sub>2</sub> nitroreductase only.
+
NemA= 4-NO2 nitroreductase only.
 
|
 
|
 
   
 
   
Line 168: Line 437:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain:  
+
*Substrain: SOS-R1
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''uvrB''
+
*Genotype of Reference Strain: ''SOS-R1 pYfkO ada<sup>+</sup> ogt<sup>+</sup>''
−
*Genotype of Experimental Strain : ''uvrB(del)''
+
*Genotype of Experimental Strain : ''SOS-R1 pYfkO ada(del) ogt(del)''
 
|
 
|
−
*Reference Condition: CB1954 metabolism by Yfko (Bs)
+
*Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 
|
 
|
−
OMP:0000172
+
OMP:0005023
 
|
 
|
−
response to chemical stimulus phenotype
+
presence of an SOS response
 
|
 
|
−
 
+
ECO:0000297
 
|
 
|
−
 
+
LacZ transcript localization evidence
 
|
 
|
−
Deletion of the uvrB nucleotide excision repair gene heightened the SOS chromatest sensitivity;  
+
Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response;  
−
Reference figure 2;
+
Reference figure 2;  
−
Yfko (Bs)= 4-NO<sub>2</sub> nitroreductase only, from B. licheniformis
+
Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
 
|
 
|
 
   
 
   

Revision as of 12:27, 26 February 2015

Citation

Prosser, GA, Patterson, AV and Ackerley, DF (2010) uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.J. Biotechnol. 150:190-4

Abstract

CB1954 is an anti-cancer prodrug that can be reduced at either of two nitro groups to form cytotoxic metabolites. We describe here two efficient and previously uncharacterized nitroreductases, YfkO from Bacillus subtilis which reduces CB1954 exclusively at the 4-NO(2) position, and NfsA from Klebsiella pneumoniae which preferentially reduces the 2-NO(2) group. Utilizing these novel enzymes, together with three previously characterized nitroreductases, we demonstrate that the Escherichia coli SOS-chromotest assay can differentially detect the 4-nitro versus 2-nitro reduction products of CB1954 following deletion of the nucleotide excision repair gene uvrB, but not mismatch repair (mutS) or methyltransferase (ada/ogt) genes. These findings may hold significance for identification and selection of nitroreductases for CB1954-mediated gene therapy, particularly when targeting tumors that are deficient in nucleotide excision repair. Moreover, we demonstrate that comparative SOS chromotest analysis in wild type and uvrB mutant strains can be used to determine whether or not nucleotide excision repair plays a significant role in processing DNA damage resulting from activation of different nitroaromatic prodrugs.

Links

PubMed Online version:10.1016/j.jbiotec.2010.08.007

Keywords

Antineoplastic Agents; Aziridines; Bacillus subtilis; Bacterial Proteins; DNA Helicases; Escherichia coli Proteins; Gene Deletion; Kinetics; Klebsiella pneumoniae; Metronidazole; Molecular Conformation; Molecular Sequence Data; Nitrofurazone; Nitroreductases; SOS Response (Genetics)

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA uvrB+
  • Genotype of Experimental Strain : SOS-R1 pNfsA uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromotest response; Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA uvrB+
  • Genotype of Experimental Strain : SOS-R1 pNfsA uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromotest response; Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsB uvrB+
  • Genotype of Experimental Strain : SOS-R1 pNfsB uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the uvrB nucleotide excision repair gene did not affect the SOS chromotest response; Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNemA uvrB+
  • Genotype of Experimental Strain : SOS-R1 pNemA uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005020

increased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NemA= 4-NO2 nitroreductase only.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pYfkO uvrB+
  • Genotype of Experimental Strain : SOS-R1 pYfkO uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005020

increased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA mutS+
  • Genotype of Experimental Strain : SOS-R1 pNfsA mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response; Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA mutS+
  • Genotype of Experimental Strain : SOS-R1 pNfsA mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response; Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsB mutS+
  • Genotype of Experimental Strain : SOS-R1 pNfsB mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response; Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNemA mutS+
  • Genotype of Experimental Strain : SOS-R1 pNemA mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response; Reference figure 2; NemA= 4-NO2 nitroreductase only.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pYfkO mutS+
  • Genotype of Experimental Strain : SOS-R1 pYfkO mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the mutS mismatch repair gene did not affect the SOS chromotest response; Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pNfsA ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response; Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pNfsA ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response; Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsB ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pNfsB ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response; Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNemA ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pNemA ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response; Reference figure 2; NemA= 4-NO2 nitroreductase only.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pYfkO ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pYfkO ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954

OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Deletion of the ada and ogt DNA methyltransferase genes did not affect the SOS chromotest response; Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis


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Notes

References

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