Difference between revisions of "Plate bioassay for antimicrobial activity"
(→Purpose) |
(→Materials Needed) |
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== Materials Needed == | == Materials Needed == | ||
| + | (For testing four plates) | ||
| + | |||
| + | 8 NBY Agar plates | ||
| + | |||
| + | 25-30 mL NBY Top Agar | ||
| + | |||
| + | 750 mL water | ||
| + | |||
| + | Sterile toothpicks | ||
| + | |||
| + | Millimeter measuring stick | ||
| + | |||
| + | Indicator Fungus-''Geotrichum candidum'' | ||
| + | |||
| + | 30 C incubator | ||
| + | |||
| + | 65 C incubator | ||
== Protocol == | == Protocol == | ||
Revision as of 13:38, 14 July 2015
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Contents
Purpose
This assay can be used to test for production of or sensitivity to antimicrobial agents by measuring a zone of inhibition around an antimicrobial compound.
Materials Needed
(For testing four plates)
8 NBY Agar plates
25-30 mL NBY Top Agar
750 mL water
Sterile toothpicks
Millimeter measuring stick
Indicator Fungus-Geotrichum candidum
30 C incubator
65 C incubator
Protocol
Notes
Papers where this or a similar method has been used
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Related Ontology Terms
References
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