Difference between revisions of "PMID:330498"

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'''Komeda, Y, Silverman, M and Simon, M'''  (1977) Genetic analysis of Escherichia coli K-12 region I flagellar mutants. ''J. Bacteriol.'' '''131''':801-8
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!align=left align='left' bgcolor='#CCCCFF' |Abstract
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Flagellar mutants in Escherichia coli region I were obtained by selection for resistance to the flagellotropic phage chi. F' elements carrying this region of the E. coli genome were then constructed. Stable merodiploid strains with a flagellar defect on the exogenate and another on the endogenote were prepared. These merodiploids yielded information on the complementation behavior of mutations in this region. Region I was shown to include at least six cistrons, flaV, flaK, flaL, flaM, flaS, and flaT. Mu-induced and deletion fla mutants were also isolated. By using these mutant strains, the transcriptional order was shown to be flaV-flaK-flaL-flaM-flaS-flaT. The definition of region I fla genes and their transcriptional relationships were confirmed by genetic tests with hybrid A phage carrying fla genes in this region.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=330498 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC235534 PMC235534]
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!align=left align='left' bgcolor='#CCCCFF' |Keywords
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Chromosome Mapping; Coliphages/growth & development; Escherichia coli/ultrastructure; Flagella/ultrastructure; Genes; Genetic Complementation Test; Lysogeny; Mutation; Operon; Transduction, Genetic
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 15:01, 16 June 2016

Citation

Komeda, Y, Silverman, M and Simon, M (1977) Genetic analysis of Escherichia coli K-12 region I flagellar mutants. J. Bacteriol. 131:801-8

Abstract

Flagellar mutants in Escherichia coli region I were obtained by selection for resistance to the flagellotropic phage chi. F' elements carrying this region of the E. coli genome were then constructed. Stable merodiploid strains with a flagellar defect on the exogenate and another on the endogenote were prepared. These merodiploids yielded information on the complementation behavior of mutations in this region. Region I was shown to include at least six cistrons, flaV, flaK, flaL, flaM, flaS, and flaT. Mu-induced and deletion fla mutants were also isolated. By using these mutant strains, the transcriptional order was shown to be flaV-flaK-flaL-flaM-flaS-flaT. The definition of region I fla genes and their transcriptional relationships were confirmed by genetic tests with hybrid A phage carrying fla genes in this region.

Links

PubMed PMC235534

Keywords

Chromosome Mapping; Coliphages/growth & development; Escherichia coli/ultrastructure; Flagella/ultrastructure; Genes; Genetic Complementation Test; Lysogeny; Mutation; Operon; Transduction, Genetic

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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