PMID:11435407

Citation	Bochner, BR, Gadzinski, P and Panomitros, E (2001) Phenotype microarrays for high-throughput phenotypic testing and assay of gene function.Genome Res. 11:1246-55
Abstract	The bacterium Escherichia coli is used as a model cellular system to test and validate a new technology called Phenotype MicroArrays (PMs). PM technology is a high-throughput technology for simultaneous testing of a large number of cellular phenotypes. It consists of preconfigured well arrays in which each well tests a different cellular phenotype and an automated instrument that continuously monitors and records the response of the cells in all wells of the arrays. For example, nearly 700 phenotypes of E. coli can be assayed by merely pipetting a cell suspension into seven microplate arrays. PMs can be used to directly assay the effects of genetic changes on cells, especially gene knock-outs. Here, we provide data on phenotypic analysis of six strains and show that we can detect expected phenotypes as well as, in some cases, unexpected phenotypes.
Links	PubMed Online version:10.1101/gr.186501
Keywords	Escherichia coli; Gene Deletion; Cellular Respiration; Phenomics; Mutation; Oligonucleotide Array Sequence Analysis; Phenotype; Phenotype Microarray
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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

• Phenotype Microarrays
  • ES- measuring carbon catabolism
  • EN- measuring nitrogen catabolism
  • EPS- measuring phosphate and sulfur catabolism
  • EA- measuring auxotrophy (growth stimulation by nutrients)
  • ES1, 2, and 3- measuring alterations in sensitivity or resistance to toxic chemicals
• OmniLog instrument
  • read and record the color change
• Color CCD camera
  • provide quantitative and kinetic information about the response of the cells

Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli		fb753	xylA	abolished utilization of xylose as a C source absent cellular respiration using xylose	Metabolic Activity	Biolog phenotype microarray	Biochemical Assay	Figure 2
Escherichia coli		fb753	xylA	abolished utilization of maltose as a C source absent cellular respiration using maltose	Metabolic Activity	Biolog phenotype microarray	Biochemical Assay	Figure 2
Escherichia coli		fb753	xylA	abolished utilization of maltotriose as a C source absent cellular respiration using maltotriose	Metabolic Activity	Biolog phenotype microarray	Biochemical Assay	Figure 2
Escherichia coli	NCBI:83333	EP014	ynjB::Tn10	abolished utilization of L-arginine as a N source	Metabolic Activity	Biolog phenotype microarray	Biochemical Assay	Figure 2
Escherichia coli	NCBI:83333	EP014	ynjB::Tn10	abolished utilization of ornithine as a N source	Metabolic Activity	Biolog phenotype microarray	Biochemical Assay	Figure 2
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References

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