Difference between revisions of "PMID:20473289"
(New PMID: Page!) |
(Fill PMID: Page!) |
||
| Line 1: | Line 1: | ||
| − | + | {{RightTOC}} | |
| + | |||
| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.13746.T57e1afc7450e3--> | ||
| + | <!-- | ||
| + | ****************************************************************************************** | ||
| + | * | ||
| + | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
| + | * | ||
| + | ****************************************************************************************** --> | ||
| + | {| id="T57e1afc7450e3" class=" tableEdit PMID_info_table" | ||
| + | |||
| + | |- | ||
| + | !align=left align='left' bgcolor='#CCCCFF' |Citation | ||
| + | || | ||
| + | '''Kim, DU, Hayles, J, Kim, D, Wood, V, Park, HO, Won, M, Yoo, HS, Duhig, T, Nam, M, Palmer, G, Han, S, Jeffery, L, Baek, ST, Lee, H, Shim, YS, Lee, M, Kim, L, Heo, KS, Noh, EJ, Lee, AR, Jang, YJ, Chung, KS, Choi, SJ, Park, JY, Park, Y, Kim, HM, Park, SK, Park, HJ, Kang, EJ, Kim, HB, Kang, HS, Park, HM, Kim, K, Song, K, Song, KB, Nurse, P and Hoe, KL''' (2010) Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe. ''Nat. Biotechnol.'' '''28''':617-23 | ||
| + | |- | ||
| + | !align=left align='left' bgcolor='#CCCCFF' |Abstract | ||
| + | || | ||
| + | We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast--the only other eukaryote for which a comprehensive knockout library exists--revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth. | ||
| + | |- | ||
| + | !align=left align='left' bgcolor='#CCCCFF' |Links | ||
| + | || | ||
| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20473289 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962850 PMC3962850] | ||
| + | Online version:[http://dx.doi.org/10.1038/nbt.1628 10.1038/nbt.1628] | ||
| + | |- | ||
| + | !align=left align='left' bgcolor='#CCCCFF' |Keywords | ||
| + | || | ||
| + | Diploidy; Gene Deletion; Genes, Essential/genetics; Genes, Fungal/genetics; Genome, Fungal/genetics; Haploidy; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Schizosaccharomyces/genetics; Schizosaccharomyces/growth & development; Sequence Homology, Nucleic Acid; Species Specificity | ||
| + | |||
| + | |- class="tableEdit_footer" | ||
| + | |<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.13746.T57e1afc7450e3&page=13746&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> || | ||
| + | |} | ||
| + | <noinclude> | ||
| + | <!-- OMP category tags --> | ||
| + | </noinclude> | ||
| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.13746.T57e1afc7450e3--> | ||
| + | |||
| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
| + | |||
| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
| + | |||
| + | ==Phenotype Annotations== | ||
| + | {{AnnotationTableHelp}} | ||
| + | <protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.13746.C57e1afc76281a--> | ||
| + | <!-- | ||
| + | ****************************************************************************************** | ||
| + | * | ||
| + | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
| + | * | ||
| + | ****************************************************************************************** --> | ||
| + | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C57e1afc76281a" class=" tableEdit Phenotype_Table_2" | ||
| + | |- align='left' bgcolor='#CCCCFF' | ||
| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
| + | |||
| + | |- class="tableEdit_footer" | ||
| + | |<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.13746.C57e1afc76281a&page=13746&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || || | ||
| + | |} | ||
| + | <noinclude> | ||
| + | <!-- OMP category tags --> | ||
| + | </noinclude> | ||
| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.13746.C57e1afc76281a--></protect> | ||
| + | |||
| + | ==Notes== | ||
| + | |||
| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
| + | |||
| + | |||
| + | [[Category:Publication]] | ||
Latest revision as of 16:53, 20 September 2016
| Citation |
Kim, DU, Hayles, J, Kim, D, Wood, V, Park, HO, Won, M, Yoo, HS, Duhig, T, Nam, M, Palmer, G, Han, S, Jeffery, L, Baek, ST, Lee, H, Shim, YS, Lee, M, Kim, L, Heo, KS, Noh, EJ, Lee, AR, Jang, YJ, Chung, KS, Choi, SJ, Park, JY, Park, Y, Kim, HM, Park, SK, Park, HJ, Kang, EJ, Kim, HB, Kang, HS, Park, HM, Kim, K, Song, K, Song, KB, Nurse, P and Hoe, KL (2010) Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 28:617-23 |
|---|---|
| Abstract |
We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast--the only other eukaryote for which a comprehensive knockout library exists--revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth. |
| Links |
PubMed PMC3962850 Online version:10.1038/nbt.1628 |
| Keywords |
Diploidy; Gene Deletion; Genes, Essential/genetics; Genes, Fungal/genetics; Genome, Fungal/genetics; Haploidy; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Schizosaccharomyces/genetics; Schizosaccharomyces/growth & development; Sequence Homology, Nucleic Acid; Species Specificity |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
