Difference between revisions of "PMID:1100857"
(→Materials and Methods Used) |
(→Materials and Methods Used) |
||
| Line 47: | Line 47: | ||
{{LitMaterials}} | {{LitMaterials}} | ||
* For the isolation of non-chemotactic mutants | * For the isolation of non-chemotactic mutants | ||
| + | **preformed liquid gradient technique (described in detail) | ||
**Diethylsulfate mutagenesis | **Diethylsulfate mutagenesis | ||
**negative swarm selection on Tryptone semi-solid medium (0.3% agar) | **negative swarm selection on Tryptone semi-solid medium (0.3% agar) | ||
| − | + | #culture spotted on plate | |
| + | #overnight incubation (wt bacteria swarmed outward from the inoculum, leaving a dense population at the center | ||
| + | #sample taken from the center | ||
| + | #perform 10 such serial selections | ||
| + | #grow samples in nutrient broth and plate on Tryptone semisolid medium | ||
| + | #200 non-swarming colonies were picked and screened for motility by examination with a microscope | ||
*For complementations and strain constructions | *For complementations and strain constructions | ||
Revision as of 14:40, 27 October 2010
| Citation |
Aswad, D and Koshland, DE (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants.J. Mol. Biol. 97:225-35 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium; Species Specificity |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
- Isolation of non-chemotactic mutants
- Description of the preformed liquid gradient technique
- utilizes a preformed gradient in a vertical column of liquid medium
- separates wild-type, non-motile, and non-motile but chemotactic into 3 regions
- None of the 72 mutants isolated exhibited a frequency of spontaneous tumbling
- 68 exhibited smooth co-ordinated motility with no evidence of tumbling
- 4 exhibited uncoordinated motility
- Results suggest there may be as many as 6 genetically distinct classes of che mutants in Salmonella
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- For the isolation of non-chemotactic mutants
- preformed liquid gradient technique (described in detail)
- Diethylsulfate mutagenesis
- negative swarm selection on Tryptone semi-solid medium (0.3% agar)
- culture spotted on plate
- overnight incubation (wt bacteria swarmed outward from the inoculum, leaving a dense population at the center
- sample taken from the center
- perform 10 such serial selections
- grow samples in nutrient broth and plate on Tryptone semisolid medium
- 200 non-swarming colonies were picked and screened for motility by examination with a microscope
- For complementations and strain constructions
- Alternative method of abortive transduction
- Strains
- all strains were derived from Salmonella typhimurium LT2
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
