Difference between revisions of "PMID:14168696"
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***shuts off the production of all 3 enzymic activities of the ''lac'' operon | ***shuts off the production of all 3 enzymic activities of the ''lac'' operon | ||
| − | * | + | *Types of ''z<sup>-</sup>y<sup>+</sup>'' revertants found in the melibiose-agar plate selection |
| + | **Deletions within the ''lac'' region | ||
| + | ***abolish basal level of β-galactosidase present in ''o<sup>o</sup>'' mutants | ||
| + | ***do not further revert to ''lac<sup>+</sup> phenotype | ||
| + | **Mutations at unlinked suppressor loci | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 15:34, 22 November 2010
| Citation |
BECKWITH, JR (1964) A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.J. Mol. Biol. 8:427-30 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Deletion; Lac; Operator; Escherichia coli; Galactosidases; Genetics; Membrane Transport Proteins; Mutation; RNA, Bacterial; Research; Enzymes; Revertants; Constitutive |
| edit table |
Main Points of the Paper
- The lac operator does not lie in the same region in which oo mutants are found.
Materials and Methods Used
- Mutant Classes
- oc mutants
- lost the sensitivity to repressor, therefore expressing β-galactosidase constitutively in the presence of the repressor
- very closely linked to z- mutations mapped at the beginning of the z gene
- oo mutants
- shuts off the production of all 3 enzymic activities of the lac operon
- oc mutants
- Types of z-y+ revertants found in the melibiose-agar plate selection
- Deletions within the lac region
- abolish basal level of β-galactosidase present in oo mutants
- do not further revert to lac+ phenotype
- Mutations at unlinked suppressor loci
- Deletions within the lac region
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
oc |
constitutive expression of β-galactosidase in the presence of repressor |
Metabolic Activity |
Biochemical Assay |
|||
|
Escherichia coli |
NCBI:562 |
oo |
constitutive repression of all 3 enzymic activities of the lac operon |
Metabolic Activity |
Biochemical Assay |
|||
|
Escherichia coli |
NCBI:562 |
oo2-M116 |
loss of repressibility |
Metabolic Activity |
Biochemical Assay |
Table 1 | ||
| edit table |
</protect>
