PMID:12769856

Citation	Morimatsu, K and Kowalczykowski, SC (2003) RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair.Mol. Cell 11:1337-47
Abstract	Genetic evidence suggests that the RecF, RecO, and RecR (RecFOR) proteins participate in a common step of DNA recombination and repair, yet the biochemical event requiring collaboration of all three proteins is unknown. Here, we show that the concerted action of the RecFOR complex directs the loading of RecA protein specifically onto gapped DNA that is coated with single-stranded DNA binding (SSB) protein, thereby accelerating DNA strand exchange. The RecFOR complex recognizes the junction between the ssDNA and dsDNA regions and requires a base-paired 5' terminus at the junction. Thus, the RecFOR complex is a structure-specific mediator that targets recombinational repair to ssDNA-dsDNA junctions. This reaction reconstitutes the initial steps of recombinational gapped DNA repair and uncovers an event also common to the repair of ssDNA-tailed intermediates of dsDNA-break repair. We propose that the behavior of the RecFOR proteins is mimicked by functional counterparts that exist in all organisms.
Links	PubMed
Keywords	Bacterial Proteins; Cell Nucleus; DNA; DNA Damage; DNA Repair; DNA, Single-Stranded; DNA-Binding Proteins; Escherichia coli Proteins; Eukaryotic Cells; Gene Rearrangement; Nucleoproteins; Prokaryotic Cells; Rec A Recombinases
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: GW8017 NCBI Taxon ID: 83333	Genotype of Reference Strain: umuD'CN32W Genotype of Experimental Strain : umuD'CN32A	Reference Condition:					By mutating the amino acid ala at the 32 position, the umuD'C is deleted, causing hypersensitivity to UV radiation.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: GW8017 NCBI Taxon ID: 83333	Genotype of Reference Strain: umuD'CN34W Genotype of Experimental Strain : umuD'CN34A	Reference Condition:					By mutating the amino acid ala at the 34 position, the umuD'C is deleted, causing hypersensitivity to UV radiation.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: GW8017 NCBI Taxon ID: 83333	Genotype of Reference Strain: umuD'CN32W Genotype of Experimental Strain : umuD'CN32A	Reference Condition:					By mutating the amino acid ala at the 32 position, the umuD'C is deleted, causing hypersensitivity to UV radiation.
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