Difference between revisions of "PMID:5327656"
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== Materials and Methods Used == | == Materials and Methods Used == | ||
| − | + | *Parent Strain: ''Δlac su<sup>-</sup>''/ F''i<sup>S</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup> | |
| + | **''i<sup>S</sup>''- dominant over ''i<sup>+</sup> and not inducible by lactose sz 43 | ||
| + | *5 strains with suppressor mutations | ||
| + | **su<sup>-</sup> | ||
| + | **su<sup>+1</sup> | ||
| + | **su<sup>+2</sup> | ||
| + | **su<sup>+3</sup> | ||
| + | **su<sup>+4</sup> | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 17:43, 16 December 2010
| Citation |
Bourgeois, S, Cohn, M and Orgel, LE (1965) Suppression of and complementation among mutants of the regulatory gene of the lactose operon of Escherichia coli.J. Mol. Biol. 14:300-2 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Escherichia coli; Genes; Genetic Code; Lactose; Molecular Biology; Mutation |
| edit table |
Main Points of the Paper
- The regulatory gene, i, codes for a protein whereas the operator gene, o, probably does not
Materials and Methods Used
- Parent Strain: Δlac su-/ FiSo+z+y+
- iS- dominant over i+ and not inducible by lactose sz 43
- 5 strains with suppressor mutations
- su-
- su+1
- su+2
- su+3
- su+4
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
Δlac su- /Fiso+z+y+ |
deficient in lactose metabolism (lac-) |
Metabolic Activity |
Biochemical Assay |
LacZ assay | ||
| edit table |
</protect>
