Difference between revisions of "PMID:6394957"
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| + | {| id="T4f7b6f8936f94" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Boeke, JD, LaCroute, F and Fink, GR''' (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.''Mol. Gen. Genet.'' '''197''':345-6 | ||
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| + | !align=left |Abstract | ||
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| + | Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6394957 PubMed] | ||
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| + | !align=left |Keywords | ||
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| + | Carboxy-Lyases; Drug Resistance, Microbial; Fungal Proteins; Microbiological Techniques; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Saccharomyces cerevisiae | ||
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| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.2904.T4f7b6f8936f94--> | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | {| id="Z4f7b6f896dc07" class=" tableEdit Phenotype_Table_2" | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Revision as of 16:45, 3 April 2012
| Citation |
Boeke, JD, LaCroute, F and Fink, GR (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.Mol. Gen. Genet. 197:345-6 |
|---|---|
| Abstract |
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells. |
| Links | |
| Keywords |
Carboxy-Lyases; Drug Resistance, Microbial; Fungal Proteins; Microbiological Techniques; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Saccharomyces cerevisiae |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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</protect>
Notes
References
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