PMID:4882748

Citation	Carter, JR, Fox, CF and Kennedy, EP (1968) Interaction of sugars with the membrane protein component of the lactose transport system of Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 60:725-32
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Bacterial Proteins; Binding Sites; Biological Transport, Active; Carbohydrates; Carbon Isotopes; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Formaldehyde; Galactose; Glycosides; Lactose
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Main Points of the Paper

• The rate of the transport system in intact cells by NEM follows apparent first-order kinetics
  • M-TDG reaction: K_D=5x10^-5 M
• The rate of the transport system in cell-free fractions by NEM was similar to that in intact cells
• Melibiose acts similarly to TDG in the NEM labelling, except that the affinity of the sugar for the protein is lower
• Allolactose had a distinct protective effect

Materials and Methods Used

• Parent Strain: ML308 (i^-z⁺y⁺a⁺)
• Membrane vesicle isolation
• TDG protection
• NEM labelling of LacY

Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli	NCBI:562	ML308-225	lacZ	Abolished beta-galactosidase activity Abolished utilization of carbon source (lactose) Abolished lactose catabolic process	Metabolic Activity	beta-galactosidase negative	Biochemical Assay	LacZ assay
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