PMID:783128

Citation	Manning, PA and Reeves, P (1976) Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants.J. Bacteriol. 127:1070-9
Abstract	Two classes of mutants, con and tolG, that appeared to be very similar in a number of respects have been shown to be identical and cotransducible with pyrD. By diethylaminoethyl-cellulose chromatography of the outer membranes, we have shown that the mutants are missing only protein 3A and retain protein 3B. Using con mutants, we were thus able to identify protein 3B on the pH 7.2 gel system of Maizel where it runs separately from protein 3A if unheated samples are used. tolG mutants were shown to be identical to con mutants in being conjugation defective with most F-like plasmid donors but not with I-like plasmid donors, and in their resistance pattern to bacteriophages and colicins. During the course of this study, it was observed that the bacteriocin produced by Serratia marcescenc JF246 was identical in its activity spectrum to colicin L-398 and is now considered to be a colicin of type L.
Links	PubMed
Keywords	Anti-Bacterial Agents; Bacterial Proteins; Cell Membrane; Chromosome Mapping; Chromosomes, Bacterial; Colicins; Coliphages; Drug Resistance, Microbial; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Mutation; Plasmids; Transduction, Genetic
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P460 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompA Genotype of Experimental Strain : 'ompA	Reference Condition:	OMP:0000038	bacteriophage-host interaction phenotype	ECO:0000182	in vitro culture assay data	Resistance to Bacteriocin JF246, table 3
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