Difference between revisions of "PMID:4577939"
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*Parent Strain: X71 (''i<sup>-</sup>z<sup>+</sup>y<sup>+</sup>a<sup>-</sup>, proC<sup>-</sup>, try<sup>-</sup>, B<sub>1</sub><sup>-</sup>'') | *Parent Strain: X71 (''i<sup>-</sup>z<sup>+</sup>y<sup>+</sup>a<sup>-</sup>, proC<sup>-</sup>, try<sup>-</sup>, B<sub>1</sub><sup>-</sup>'') | ||
*Additional Strain: X9003 (''i<sup>+</sup>z<sup>-</sup>y<sup>+</sup>a<sup>+</sup>, B<sub>1</sub><sup>-</sup>'') | *Additional Strain: X9003 (''i<sup>+</sup>z<sup>-</sup>y<sup>+</sup>a<sup>+</sup>, B<sub>1</sub><sup>-</sup>'') | ||
| − | *Additional Strain: X71-54 (''i<sup>-</sup>z<sup>+</sup>y<sup>UN</sup>a<sup>-</sup>'') | + | *Additional Strain: X71-54 (''i<sup>-</sup>z<sup>+</sup>y<sup>UN</sup>a<sup>-</sup>'')- energy uncoupled mutant |
| − | *Additional Strain: 316 (''i<sup>-</sup>z<sup>+</sup>y<sup>-</sup>a<sup>-</sup>'') | + | *Additional Strain: 316 (''i<sup>-</sup>z<sup>+</sup>y<sup>-</sup>a<sup>-</sup>'')- ''y<sup>-</sup>'' strain |
| + | |||
| + | *β-galactosidase assay | ||
| + | *α-galactosidase assay | ||
| + | *Carrier-mediated transport assay | ||
| + | *thiogalactoside transacetylase activity was determined | ||
| + | |||
| + | *P<sub>1</sub> Transduction | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 11:36, 13 December 2010
| Citation |
Kusch, M and Wilson, TH (1973) Defective lactose utilization by a mutant of Escherichia coli energy-uncoupled for lactose transport. The advantages of active transport versus facilitated diffusion.Biochim. Biophys. Acta 311:109-22 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Biological Transport; Biological Transport, Active; Carbon Isotopes; Cell Division; Densitometry; Diffusion; Disaccharides; Enzyme Induction; Escherichia coli; Galactosidases; Kinetics; Lactose; Mutation; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
- Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-)
- Additional Strain: X9003 (i+z-y+a+, B1-)
- Additional Strain: X71-54 (i-z+yUNa-)- energy uncoupled mutant
- Additional Strain: 316 (i-z+y-a-)- y- strain
- β-galactosidase assay
- α-galactosidase assay
- Carrier-mediated transport assay
- thiogalactoside transacetylase activity was determined
- P1 Transduction
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
energy uncoupled mutant |
||||
|
Escherichia coli |
NCBI:562 |
316 |
lacY- |
abolished transporter activity |
| |||
| edit table |
</protect>
