Difference between revisions of "PMID:357427"
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+ | {| id="F4f9efe52abbdc" class=" tableEdit PMID_info_table" | ||
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+ | |- | ||
+ | !align=left |Citation | ||
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+ | '''Lipsett, MN''' (1978) Enzymes producing 4-thiouridine in Escherichia coli tRNA: approximate chromosomal locations of the genes and enzyme activities in a 4-thiouridine-deficient mutant.''J. Bacteriol.'' '''135''':993-7 | ||
+ | |- | ||
+ | !align=left |Abstract | ||
+ | || | ||
+ | A previously described mutant of Escherichia coli which lacks 4-thiouridine in its tRNA was here shown to be deficient in factor A, one of the two proteins responsible for this thiolation of uridine. Addition of exogenous factor A restored the thiolating ability of extracts prepared from the mutant. The activities of the two thiolation proteins were governed by genes at two widely separated positions on the chromosome, as determined with F-prime merodiploids. The site governing factor A activity lay roughly in the region of the recently reported position of nuv, a gene controlling the production of 4-thiouridine in tRNA. | ||
+ | |- | ||
+ | !align=left |Links | ||
+ | || | ||
+ | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=357427 PubMed] | ||
+ | |||
+ | |- | ||
+ | !align=left |Keywords | ||
+ | || | ||
+ | Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli; Genes; Mutation; RNA, Bacterial; RNA, Transfer; Thiouridine | ||
+ | |||
+ | |- class="tableEdit_footer" | ||
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+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3083.F4f9efe52abbdc--> | ||
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+ | ==Main Points of the Paper == | ||
+ | {{LitSignificance}} | ||
+ | |||
+ | == Materials and Methods Used == | ||
+ | {{LitMaterials}} | ||
+ | |||
+ | ==Phenotype Annotations== | ||
+ | {{AnnotationTableHelp}} | ||
+ | <protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.3083.W4f9efe52ba1d7--> | ||
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+ | {| id="W4f9efe52ba1d7" class=" tableEdit Phenotype_Table_2" | ||
+ | |- | ||
+ | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
+ | |||
+ | |- class="tableEdit_footer" | ||
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+ | |} | ||
+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3083.W4f9efe52ba1d7--></protect> | ||
+ | |||
+ | ==Notes== | ||
+ | |||
+ | ==References== | ||
+ | {{RefHelp}} | ||
+ | <references/> | ||
+ | |||
+ | |||
+ | [[Category:Publication]] |
Revision as of 16:04, 30 April 2012
Citation |
Lipsett, MN (1978) Enzymes producing 4-thiouridine in Escherichia coli tRNA: approximate chromosomal locations of the genes and enzyme activities in a 4-thiouridine-deficient mutant.J. Bacteriol. 135:993-7 |
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Abstract |
A previously described mutant of Escherichia coli which lacks 4-thiouridine in its tRNA was here shown to be deficient in factor A, one of the two proteins responsible for this thiolation of uridine. Addition of exogenous factor A restored the thiolating ability of extracts prepared from the mutant. The activities of the two thiolation proteins were governed by genes at two widely separated positions on the chromosome, as determined with F-prime merodiploids. The site governing factor A activity lay roughly in the region of the recently reported position of nuv, a gene controlling the production of 4-thiouridine in tRNA. |
Links | |
Keywords |
Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli; Genes; Mutation; RNA, Bacterial; RNA, Transfer; Thiouridine |
edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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</protect>
Notes
References
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