Difference between revisions of "PMID:4933690"
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== Materials and Methods Used == | == Materials and Methods Used == | ||
| − | + | *Parent Strain: X71 (''i<sup>-</sup>z<sup>+</sup>y<sup>+</sup>a<sup>-</sup>, proC<sup>-</sup>, try<sup>-</sup>'', B<sub>1</sub><sup>-</sup>, F<sup>-</sup>) | |
| + | *Additional Strain: X71-54 | ||
| + | *Additional Strain: Ca77 (lactose-deleted, B<sub>1</sub><sup>-</sup>, Hfr) | ||
| + | |||
| + | *β-galactosidase assay | ||
| + | *ONPG uptake assay | ||
| + | *Thiomethylgalactoside (TMG) transport assay | ||
| + | *P<sub>1</sub> Transduction | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 14:39, 13 December 2010
| Citation |
Wilson, TH, Kusch, M and Kashket, ER (1970) A mutant in Escherichia coli energy-uncoupled for lactose transport: a defect in the lactose-operon.Biochem. Biophys. Res. Commun. 40:1409-14 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Biological Transport, Active; Carbon Isotopes; Cell Membrane; Coliphages; Escherichia coli; Galactose; Glycosides; Lactose; Molecular Biology; Mutation; Nitrobenzenes; Operon; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
- Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-, F-)
- Additional Strain: X71-54
- Additional Strain: Ca77 (lactose-deleted, B1-, Hfr)
- β-galactosidase assay
- ONPG uptake assay
- Thiomethylgalactoside (TMG) transport assay
- P1 Transduction
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
