Difference between revisions of "PMID:6355059"
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+ | {| id="U4fb69b50a8a0c" class=" tableEdit PMID_info_table" | ||
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+ | |- | ||
+ | !align=left |Citation | ||
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+ | '''Stalmach, ME, Grothe, S and Wood, JM''' (1983) Two proline porters in Escherichia coli K-12.''J. Bacteriol.'' '''156''':481-6 | ||
+ | |- | ||
+ | !align=left |Abstract | ||
+ | || | ||
+ | Escherichia coli mutants defective at putP and putA lack proline transport via proline porter I and proline dehydrogenase activity, respectively. They retain a proline uptake system (proline porter II) that is induced during tryptophan-limited growth and are sensitive to the toxic L-proline analog, 3,4-dehydroproline. 3,4-Dehydroproline-resistant mutants derived from a putP putA mutant lack proline porter II. Auxotrophic derivatives derived from putP+ or putP bacteria can grow if provided with proline at low concentration (25 microM); those derived from the 3,4-dehydroproline-resistant mutants require high proline for growth (2.5 mM). We conclude that E. coli, like Salmonella typhimurium, possesses a second proline porter that is inactivated by mutations at the proP locus. | ||
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+ | !align=left |Links | ||
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+ | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6355059 PubMed] | ||
+ | |||
+ | |- | ||
+ | !align=left |Keywords | ||
+ | || | ||
+ | Amino Acid Transport Systems, Neutral; Escherichia coli; Genes, Bacterial; Genotype; Isoenzymes; Kinetics; Membrane Transport Proteins; Mutation; Proline; Species Specificity | ||
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+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3182.U4fb69b50a8a0c--> | ||
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+ | ==Main Points of the Paper == | ||
+ | {{LitSignificance}} | ||
+ | |||
+ | == Materials and Methods Used == | ||
+ | {{LitMaterials}} | ||
+ | |||
+ | ==Phenotype Annotations== | ||
+ | {{AnnotationTableHelp}} | ||
+ | <protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.3182.X4fb69b50ac189--> | ||
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+ | {| id="X4fb69b50ac189" class=" tableEdit Phenotype_Table_2" | ||
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+ | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
+ | |||
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+ | |} | ||
+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3182.X4fb69b50ac189--></protect> | ||
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+ | ==Notes== | ||
+ | |||
+ | ==References== | ||
+ | {{RefHelp}} | ||
+ | <references/> | ||
+ | |||
+ | |||
+ | [[Category:Publication]] |
Revision as of 13:56, 18 May 2012
Citation |
Stalmach, ME, Grothe, S and Wood, JM (1983) Two proline porters in Escherichia coli K-12.J. Bacteriol. 156:481-6 |
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Abstract |
Escherichia coli mutants defective at putP and putA lack proline transport via proline porter I and proline dehydrogenase activity, respectively. They retain a proline uptake system (proline porter II) that is induced during tryptophan-limited growth and are sensitive to the toxic L-proline analog, 3,4-dehydroproline. 3,4-Dehydroproline-resistant mutants derived from a putP putA mutant lack proline porter II. Auxotrophic derivatives derived from putP+ or putP bacteria can grow if provided with proline at low concentration (25 microM); those derived from the 3,4-dehydroproline-resistant mutants require high proline for growth (2.5 mM). We conclude that E. coli, like Salmonella typhimurium, possesses a second proline porter that is inactivated by mutations at the proP locus. |
Links | |
Keywords |
Amino Acid Transport Systems, Neutral; Escherichia coli; Genes, Bacterial; Genotype; Isoenzymes; Kinetics; Membrane Transport Proteins; Mutation; Proline; Species Specificity |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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<protect>
Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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Notes
References
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