PMID:16591520

Citation	Fox, CF, Carter, JR and Kennedy, EP (1967) GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 57:698-705
Abstract	No abstract in PubMed
Links	PubMed
Keywords
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Main Points of the Paper

• The M protein of β-galactoside transport is the product of lacY
• M is localized in the spheroplast membrane fraction of the cell
• Estimate: xx functional subunits of M protein /cell

Materials and Methods Used

• Strain: ML30 (i⁺z⁺y⁺a⁺)
• Strain: ML308-225 (i^-z^-y⁺a⁺)- a derivative of ML30
• Strain: 3000 (i⁺z⁺y⁺a⁺)
• Strain: YA470 (i⁺z⁺y^-)- y^- amber mutant
• Strain: CA7047 (i⁺z⁺y^-a⁺)- y^- amber mutant
• Strain: CA8202 (i⁺z⁺y^-a⁺)- y^- amber mutant
• Strain: CA8204 (i⁺z⁺y^-a⁺)- y^- amber mutant
• Strain: MS1054 (i⁺z⁺y⁺a⁺)- y^- deletion
• Strain: MS356 (i⁺z⁺y^-a⁺)- y^- deletion
• Strain: MS1019 (i⁺z⁺y^-a⁺)- y^- deletion
• Strain: MS1068 (i⁺z⁺y^-a⁺)- y^- deletion
• Strain: A 279A (i⁺z⁺ya⁺)

• Preparation of membrane-containing particulate fraction
• Unlabeled N-ethylmaleimide treatment
• M protein assay

Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli	NCBI:562	ML308	lacI-	Abolished negative regulation of gene expression (LacY) Constitutive gene expression	Other	membrane particulate fractions contain the M protein regardless of induction status-constitutive expression of M protein (LacY)	Other	Table 1- protein-bound NEM
Escherichia coli	NCBI:562	ML3	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 1- NEM binding
Escherichia coli	NCBI:562	ML35	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 1- NEM binding
Escherichia coli K12	NCBI:83333	YA470	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 2- NEM binding
Escherichia coli K12	NCBI:83333	CA7047	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 2- NEM binding
Escherichia coli K12	NCBI:83333	CA8202	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 2- NEM binding
Escherichia coli K12	NCBI:83333	CA8204-ts 3	lacY	Temperature-dependent (Conditional) growth Temperature-sensitive growth on lactose medium Temperature-dependent lactose catabolic process	Growth	exhibited lac+ phenotype at 25C, but appeared lac- when grown at 42C	Plating Assay	Figure 1- ONPG hydrolysis in vivo
Escherichia coli K12	NCBI:83333	MS1054	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 2- NEM binding
Escherichia coli K12	NCBI:83333	MS1068	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 2- NEM binding
Escherichia coli K12	NCBI:83333	MS356	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 2- NEM binding
Escherichia coli K12	NCBI:83333	MS1019	lacY-	absence of substrate binding	Other	no detectable M protein in membrane particulate fractions	Other	Table 2- NEM binding
Escherichia coli K12	NCBI:83333	CA8204	lacY-	Absent substrate binding Absent transporter activity Abolished lactose catabolic process	Other	no detectable M protein in membrane particulate fractions	Other	Table 2- NEM binding
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