PMID:1886615

Citation	Rakonjac, J, Milic, M and Savic, DJ (1991) cysB and cysE mutants of Escherichia coli K12 show increased resistance to novobiocin.Mol. Gen. Genet. 228:307-11
Abstract	Mutations in the cysB and cysE genes of Escherichia coli K12 cause an increase in resistance to the gyrase inhibitor novobiocin but not to coumermycin, acriflavine and rifampicin. This unusual relationship was also observed among spontaneous novobiocin resistant (Novr) mutants: 10% of Novr mutants isolated on rich (LA) plates with novobiocin could not grow on minimal plates, and among those approximately half were cysB or cysE mutants. Further analyses demonstrated that cysB and cysE negative alleles neither interfere with transport of novobiocin nor affect DNA supercoiling.
Links	PubMed
Keywords	Acetyltransferases; Acriflavine; Aminocoumarins; Bacterial Proteins; Cell Survival; Coumarins; DNA-Binding Proteins; Dose-Response Relationship, Drug; Drug Resistance, Microbial; Escherichia coli; Escherichia coli Proteins; Mutation; Novobiocin; Rifampin; Serine O-Acetyltransferase
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: C600 NCBI Taxon ID: 83333	Genotype of Reference Strain: cysB Genotype of Experimental Strain : cysB in mutant strain SY380	Reference Condition:					Resistance to Novobiocin
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1157 NCBI Taxon ID: 83333	Genotype of Reference Strain: cysB Genotype of Experimental Strain : cysB(TS)	Reference Condition:					Resistance to Novobiocin. Permissive temperature 30C exhibited wild type levels of resistance to Novobiocin. Temperature of 42C allowed increased resistance.
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