Difference between revisions of "PMID:4887874"

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(Table edited by Azweifel via TableEdit)
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
 
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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|-
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''Escherichia coli''
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NCBI:562
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NG545-pi(1)<sub>a</sub>
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lacZ
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able to metabolize melibiose
 +
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 +
Growth
 +
|
 +
growth on melibiose- polar effects
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|
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Plating Assay
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|
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Table 2 
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''Escherichia coli''
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NCBI:562
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NG545-pi(1)
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 +
lacZ
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 +
 +
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Growth
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 +
able to metabolize lactose only in a su<sub>I</sub><sub>+</sub> background
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Plating Assay
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Table 2- UAG polar mutation
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|-
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''Escherichia coli''
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NCBI:562
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NG545-pi(1)<sub>b</sub>
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lacZ
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 +
unable to metabolize melibiose
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 +
Growth
 +
|
 +
growth on melibiose-polar effects
 +
|
 +
Plating Assay
 +
|
 +
Table 2- 
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|-
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|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
NG545-pi(1)<sub>a</sub>
 +
|
 +
lacZ
 +
|
 +
reduced beta-galactosidase activity
 +
|
 +
Metabolic Activity
 +
|
 +
trace amounts when induced
 +
|
 +
Biochemical Assay
 +
|
 +
Table 2 
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
NG545-pi(1)<sub>b</sub>
 +
|
 +
lacZ
 +
|
 +
reduced beta-galactosidase activity
 +
|
 +
Metabolic Activity
 +
|
 +
trace amounts when induced
 +
|
 +
Biochemical Assay
 +
|
 +
Table 2 
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
NG545B
 +
|
 +
lacZ
 +
|
 +
reduced acetylase activity
 +
|
 +
Metabolic Activity
 +
|
 +
4.2% of the wild type level when induced
 +
|
 +
Biochemical Assay
 +
|
 +
Tables 3 and 4 
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
NG545-pi(1)<sub>a</sub>B
 +
|
 +
lacZ
 +
|
 +
reduced acetylase activity
 +
|
 +
Metabolic Activity
 +
|
 +
35% of the wild type level when induced
 +
|
 +
Biochemical Assay
 +
|
 +
Tables 3 and 4 
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
NG545-pi(1)<sub>b</sub>B
 +
|
 +
lacZ
 +
|
 +
reduced acetylase activity
 +
|
 +
Metabolic Activity
 +
|
 +
33.5% of the wild type level when induced
 +
|
 +
Biochemical Assay
 +
|
 +
Tables 3 and 4 
  
 
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Revision as of 17:05, 15 December 2010

Citation

Grodzicker, T and Zipser, D (1968) A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.J. Mol. Biol. 38:305-14

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Acyltransferases; Chromosome Mapping; Escherichia coli; Genes; Membrane Transport Proteins; Mutation; Operon

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Main Points of the Paper

  • π, a new site of initiation of protein synthesis is described
    • operates efficiently to restore permease and acetylase activity to a high level

Materials and Methods Used

  • Parent Strain: NG545 (trp-8, SmS Hfr)
  • Strain: CA77B (Δlac, trp-8, SmR F-)
  • Strain: X103B (Δlac pro, trp-8, SmR F-)
  • Strain: M107 (Δlac, suI+, SmR F-)
  • Strain: ΔLacW (Δlac, his-, SmR F-)
  • Strain: E29 (lac+ , F'/lac+, threonine-, leucine-, B1-, SmR)


  • Acetylase assay
  • β-galactosidase assay

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:562

NG545-pi(1)a

lacZ

able to metabolize melibiose

Growth

growth on melibiose- polar effects

Plating Assay

Table 2

Escherichia coli

NCBI:562

NG545-pi(1)

lacZ

Growth

able to metabolize lactose only in a suI+ background

Plating Assay

Table 2- UAG polar mutation

Escherichia coli

NCBI:562

NG545-pi(1)b

lacZ

unable to metabolize melibiose

Growth

growth on melibiose-polar effects

Plating Assay

Table 2-

Escherichia coli

NCBI:562

NG545-pi(1)a

lacZ

reduced beta-galactosidase activity

Metabolic Activity

trace amounts when induced

Biochemical Assay

Table 2

Escherichia coli

NCBI:562

NG545-pi(1)b

lacZ

reduced beta-galactosidase activity

Metabolic Activity

trace amounts when induced

Biochemical Assay

Table 2

Escherichia coli

NCBI:562

NG545B

lacZ

reduced acetylase activity

Metabolic Activity

4.2% of the wild type level when induced

Biochemical Assay

Tables 3 and 4

Escherichia coli

NCBI:562

NG545-pi(1)aB

lacZ

reduced acetylase activity

Metabolic Activity

35% of the wild type level when induced

Biochemical Assay

Tables 3 and 4

Escherichia coli

NCBI:562

NG545-pi(1)bB

lacZ

reduced acetylase activity

Metabolic Activity

33.5% of the wild type level when induced

Biochemical Assay

Tables 3 and 4

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</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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