Difference between revisions of "PMID:334074"
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| + | {| id="N505661fbc4f29" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Powers, EM and Latt, TG ''' (1977) Simplified 48-hour IMVic test: an agar plate method. ''Appl. Environ. Microbiol.'' '''34''':274-9 | ||
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| + | !align=left |Abstract | ||
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| + | An agar plate method was developed for the performance of the IMVic (indole, methyl red, Voges-Proskauer, and citrate) tests in lieu of the conventional tubed liquid media. By modifying the composition of the media and adding agar, a single "X"-compartmented petri dish was prepared containing all four IMVic test media. Ease of performance and simplification of the test were achieved by inoculating all four media simultaneously from a single colony (single inoculum) on eosin-methylene blue agar. Tests with 87 cultures, representing 7 genera in the family Enterobacteriaceae, were completed with typical (correct) IMVic patterns for all cultures within 48 h. Parallel tests with conventional media showed that the agar plate method was superior, more sensitive, faster, and simpler to perform, and less time was required to identify Escherichia coli by 72 h. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=334074 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC242642 PMC242642] | ||
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| + | !align=left |Keywords | ||
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| + | Bacteriological Techniques; Citrates/metabolism; Enterobacteriaceae/classification; Enterobacteriaceae/metabolism; Escherichia coli/classification; Escherichia coli/metabolism; Indoles/metabolism | ||
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| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3399.N505661fbc4f29--> | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Revision as of 18:34, 16 September 2012
| Citation |
Powers, EM and Latt, TG (1977) Simplified 48-hour IMVic test: an agar plate method. Appl. Environ. Microbiol. 34:274-9 |
|---|---|
| Abstract |
An agar plate method was developed for the performance of the IMVic (indole, methyl red, Voges-Proskauer, and citrate) tests in lieu of the conventional tubed liquid media. By modifying the composition of the media and adding agar, a single "X"-compartmented petri dish was prepared containing all four IMVic test media. Ease of performance and simplification of the test were achieved by inoculating all four media simultaneously from a single colony (single inoculum) on eosin-methylene blue agar. Tests with 87 cultures, representing 7 genera in the family Enterobacteriaceae, were completed with typical (correct) IMVic patterns for all cultures within 48 h. Parallel tests with conventional media showed that the agar plate method was superior, more sensitive, faster, and simpler to perform, and less time was required to identify Escherichia coli by 72 h. |
| Links | |
| Keywords |
Bacteriological Techniques; Citrates/metabolism; Enterobacteriaceae/classification; Enterobacteriaceae/metabolism; Escherichia coli/classification; Escherichia coli/metabolism; Indoles/metabolism |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
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