Difference between revisions of "PMID:18202367"

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'''Zhang, XX  and Rainey, PB '''  (2008) Dual involvement of CbrAB and NtrBC in the regulation of histidine utilization in Pseudomonas fluorescens SBW25. ''Genetics'' '''178''':185-95
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Pseudomonas fluorescens SBW25 is capable of growing on histidine as a sole source of carbon and/or nitrogen. Previous work showed that the two-component regulatory system CbrAB is required for expression of the histidine utilization (hut) locus when histidine is the sole source of carbon and nitrogen. Here, using mutational analysis and transcriptional assays, we demonstrate involvement of a second two-component system, NtrBC. When histidine is the sole carbon source, transcription of the hutU operon is initiated from a sigma54-type promoter and requires CbrB (an enhancer binding protein for sigma54-recruitment). However, when histidine is the sole nitrogen source, the hutU operon is transcribed from a sigma70-type promoter and requires either CbrB or the nitrogen regulator, NtrC. No role was found for the SBW25 homolog of the nitrogen assimilation control protein (NAC). Biolog phenotypic microarray analysis of the ability of the three mutants (DeltacbrB, DeltantrC, and DeltacbrB DeltantrC) to utilize 190 carbon and 95 nitrogen substrates confirmed the central regulatory roles of CbrAB and NtrBC in cellular carbon and nitrogen catabolism: deletion of cbrB abolished growth on 20 carbon substrates; deletion of ntrC eliminated growth on 28 nitrogen substrates. A double cbrB-ntrC mutant was unable to utilize a further 14 nitrogen substrates (including histidine, proline, leucine, isoleucine, and valine). Our data show that CbrAB plays a role in regulation of both carbon and nitrogen catabolism and maintains activity of catabolic pathways under different C:N ratios.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18202367 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206070 PMC2206070]
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Online version:[http://dx.doi.org/10.1534/genetics.107.081984 10.1534/genetics.107.081984]
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Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Carbon/metabolism; Gene Expression Regulation, Bacterial/drug effects; Glutamic Acid/pharmacology; Histidine/metabolism; Histidine/pharmacology; Microarray Analysis; Molecular Sequence Data; Nitrogen/metabolism; Operon/genetics; Phenotype; Pseudomonas fluorescens/drug effects; Pseudomonas fluorescens/genetics; Pseudomonas fluorescens/growth & development; Sequence Homology, Amino Acid; Transcription Initiation Site
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 11:49, 17 September 2012

Citation

Zhang, XX and Rainey, PB (2008) Dual involvement of CbrAB and NtrBC in the regulation of histidine utilization in Pseudomonas fluorescens SBW25. Genetics 178:185-95

Abstract

Pseudomonas fluorescens SBW25 is capable of growing on histidine as a sole source of carbon and/or nitrogen. Previous work showed that the two-component regulatory system CbrAB is required for expression of the histidine utilization (hut) locus when histidine is the sole source of carbon and nitrogen. Here, using mutational analysis and transcriptional assays, we demonstrate involvement of a second two-component system, NtrBC. When histidine is the sole carbon source, transcription of the hutU operon is initiated from a sigma54-type promoter and requires CbrB (an enhancer binding protein for sigma54-recruitment). However, when histidine is the sole nitrogen source, the hutU operon is transcribed from a sigma70-type promoter and requires either CbrB or the nitrogen regulator, NtrC. No role was found for the SBW25 homolog of the nitrogen assimilation control protein (NAC). Biolog phenotypic microarray analysis of the ability of the three mutants (DeltacbrB, DeltantrC, and DeltacbrB DeltantrC) to utilize 190 carbon and 95 nitrogen substrates confirmed the central regulatory roles of CbrAB and NtrBC in cellular carbon and nitrogen catabolism: deletion of cbrB abolished growth on 20 carbon substrates; deletion of ntrC eliminated growth on 28 nitrogen substrates. A double cbrB-ntrC mutant was unable to utilize a further 14 nitrogen substrates (including histidine, proline, leucine, isoleucine, and valine). Our data show that CbrAB plays a role in regulation of both carbon and nitrogen catabolism and maintains activity of catabolic pathways under different C:N ratios.

Links

PubMed PMC2206070 Online version:10.1534/genetics.107.081984

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Carbon/metabolism; Gene Expression Regulation, Bacterial/drug effects; Glutamic Acid/pharmacology; Histidine/metabolism; Histidine/pharmacology; Microarray Analysis; Molecular Sequence Data; Nitrogen/metabolism; Operon/genetics; Phenotype; Pseudomonas fluorescens/drug effects; Pseudomonas fluorescens/genetics; Pseudomonas fluorescens/growth & development; Sequence Homology, Amino Acid; Transcription Initiation Site

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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