Difference between revisions of "PMID:5324399"
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== Materials and Methods Used == | == Materials and Methods Used == | ||
{{LitMaterials}} | {{LitMaterials}} | ||
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+ | *Strain: ML30 (i+z+y+x+) | ||
+ | *Strain: ML308 (i-z+y+x+) | ||
+ | *ONPG hydrolysis | ||
+ | *thiogalactoside transacetylase assay | ||
+ | *radio-labeled substrate protein binding assay | ||
==Phenotype Annotations== | ==Phenotype Annotations== |
Latest revision as of 16:37, 18 September 2012
Citation |
Fox, CF and Kennedy, EP (1965) Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 54:891-9 |
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Abstract |
No abstract in PubMed |
Links | |
Keywords |
Bacterial Proteins; Biological Transport; Carbon Isotopes; Escherichia coli/enzymology; Ethylmaleimide/pharmacology; Galactosidases; Glycosides/metabolism |
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Main Points of the Paper
Please summarize the main points of the paper.
- Provides evidence that component M is a protein, distinct from β-galactosidase and thiogalactoside transacetylase
- M is controlled by the i gene
- M is localized to the membrane-containing particulate fraction and has a high affinity for certain β-galactosides
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- Strain: ML30 (i+z+y+x+)
- Strain: ML308 (i-z+y+x+)
- ONPG hydrolysis
- thiogalactoside transacetylase assay
- radio-labeled substrate protein binding assay
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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</protect>
Notes
References
See Help:References for how to manage references in omp dev.