Difference between revisions of "PMID:794878"

Revision as of 18:31, 1 November 2012

Citation	Gellert, M, O'Dea, MH, Itoh, T and Tomizawa, J (1976) Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.Proc. Natl. Acad. Sci. U.S.A. 73:4474-8
Abstract	Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a cell-free system is partially relieved by adding resistant DNA gyrase. Both in the case of coliclls. DNA molecules which are converted to the covalently circular form in thepresence of coumermycin remain relaxed, instead of achieving their normal supercoiled conformation. We conclude that DNA gyrase controls the supercoiling of DNA in E. coli.
Links	PubMed
Keywords	Anti-Bacterial Agents; Colicins; Coumarins; DNA Replication; DNA, Bacterial; DNA, Circular; DNA, Viral; Escherichia coli; Mutation; Novobiocin; Nucleic Acid Conformation; Plasmids; Pyrroles
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	Notes
a mutation or genetic difference within a strain	Taxon: Escherichia coli	Genotype of Reference Strain:	Reference Condition:
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Col E1 NCBI Taxon ID: 83333	Genotype of Reference Strain: gyrB⁺ Genotype of Experimental Strain : gyrB mutation	Reference Condition:	As seen in figure 1 (lanes i-n) cou^r was used to confer resistance toward Novobiocin.
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-DNA gyrase is resistant to both Novobiocin and coumermycin. In the toluenized cells of E.coli the drug inhibits the synthesis of DNA but not repair synthesis.
+a mutation or genetic difference within a strain
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-*Taxon: Coumermycin-resistant E.coli mutants. Starting strain NT525, similar to strain K12. NI708, NI741, CRT46 dnaA, NI748, N1071, YS1.
+*Taxon: Escherichia coli
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-*Genotype of Reference Strain: DNA gyrase was isolated from N99 recB21
+*Genotype of Reference Strain:
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-*Reference Condition: Strain Nt525 resistant to Novobiocin grew on tryptone agar of 600 ug/ml of Novobiocin. Coumermycin resistant isolates: NI741 and NI748 grew well in a liquid culture of 60ug/ml of coumermycin. Growth of other starins were blocked by 15ug/ml of coumermycin. Relaxed and intracellulary supercoiled samples were prepared. Relaxed and circular phage DNA was also prepared.
+*Reference Condition:
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 *Taxon: Escherichia coli
+*Strain: K-12
+*Substrain: Col E1
+*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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-*Genotype of Reference Strain:
+*Genotype of Reference Strain: ''gyrB<sup>+</sup>''
+*Genotype of Experimental Strain : ''gyrB'' mutation
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 *Reference Condition:
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+As seen in figure 1 (lanes i-n) cou<sup>r</sup> was used to confer resistance toward Novobiocin.
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