Difference between revisions of "PMID:14168696"
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*Mutant Classes | *Mutant Classes | ||
**''o<sup>c</sup>'' mutants | **''o<sup>c</sup>'' mutants | ||
| − | ***lost the sensitivity to repressor, therefore expressing | + | ***lost the sensitivity to repressor, therefore expressing β-galactosidase constitutively in the presence of the repressor |
*** very closely linked to ''z''- mutations mapped at the beginning of the ''z'' gene | *** very closely linked to ''z''- mutations mapped at the beginning of the ''z'' gene | ||
| + | |||
**''o<sup>o</sup>'' mutants | **''o<sup>o</sup>'' mutants | ||
***shuts off the production of all 3 enzymic activities of the ''lac'' operon | ***shuts off the production of all 3 enzymic activities of the ''lac'' operon | ||
Revision as of 17:31, 16 December 2010
| Citation |
BECKWITH, JR (1964) A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.J. Mol. Biol. 8:427-30 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Deletion; Lac; Operator; Escherichia coli; Galactosidases; Genetics; Membrane Transport Proteins; Mutation; RNA, Bacterial; Research; Enzymes; Revertants; Constitutive |
| edit table |
Main Points of the Paper
- The lac operator does not lie in the same region in which oo mutants are found.
Materials and Methods Used
- Mutant Classes
- oc mutants
- lost the sensitivity to repressor, therefore expressing β-galactosidase constitutively in the presence of the repressor
- very closely linked to z- mutations mapped at the beginning of the z gene
- oc mutants
- oo mutants
- shuts off the production of all 3 enzymic activities of the lac operon
- oo mutants
- Types of z-y+ revertants found in the melibiose-agar plate selection
- Deletions within the lac region
- abolish basal level of β-galactosidase present in oo mutants
- do not further revert to lac+ phenotype
- Mutations at unlinked suppressor loci
- Deletions within the lac region
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
oo |
constitutive repression of all 3 enzymic activities of the lac operon |
Expression |
Biochemical Assay |
|||
|
Escherichia coli |
NCBI:562 |
oo2-M116 |
Expression |
loss of repressibility |
Biochemical Assay |
Table 1 | ||
|
Escherichia coli |
NCBI:562 |
oc |
constitutive expression of beta-galactosidase in the presence of repressor |
Metabolic Activity |
Biochemical Assay |
|||
|
Escherichia coli |
NCBI:562 |
oo2-M12 |
no beta-galactosidase activity |
Metabolic Activity |
deletion in lac region |
Biochemical Assay |
Table 1 | |
|
Escherichia coli |
NCBI:562 |
oo2-M15 |
no beta-galactosidase activity |
Metabolic Activity |
deletion in lac region |
Biochemical Assay |
Table 1 | |
|
Escherichia coli |
NCBI:562 |
oo2-M41 |
no beta-galactosidase activity |
Metabolic Activity |
deletion in lac region |
Biochemical Assay |
Table 1 | |
| edit table |
</protect>
