Difference between revisions of "PMID:5327656"
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| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | Δ''lac su-'' /F''i<sup>S</sup>i<sup>-</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup>'' | ||
| + | | | ||
| + | ''lacI<sup>S</sup>'' | ||
| + | | | ||
| + | able to utilize lactose | ||
| + | | | ||
| + | Expression | ||
| + | | | ||
| + | 975 ''lac<sup>+</sup>'' revertants | ||
| + | | | ||
| + | |||
| + | | | ||
| + | |||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | Δ''lac su-'' /F''i<sup>S</sup>o<sup>C</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup>'' | ||
| + | | | ||
| + | ''lacO<sup>C</sup>'' | ||
| + | | | ||
| + | able to utilize lactose | ||
| + | | | ||
| + | Expression | ||
| + | | | ||
| + | 125 ''lac<sup>+</sup>'' revertants | ||
| + | | | ||
| + | |||
| + | | | ||
| + | |||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | ''lac<sup>+</sup> su<sup>+1</sup>'' /F''i<sup>S</sup>i<sup>-</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup>'' | ||
| + | | | ||
| + | ''i<sup>S</sup>i<sup>-</sup>'' | ||
| + | | | ||
| + | unable to utilize lactose | ||
| + | | | ||
| + | Expression | ||
| + | | | ||
| + | restore the ''i<sup>S</sup>'' phenotype to 10 revertants- giving rise to dominant ''lac<sup>-</sup>'' diploids in ''i<sup>+</sup>su<sup>+1</sup>'' | ||
| + | | | ||
| + | Plating Assay | ||
| + | | | ||
| + | in text, pg. 300 | ||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | ''lac<sup>+</sup> su<sup>+</sup>'' /F''i<sup>S</sup>i<sup>-</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup>'' | ||
| + | | | ||
| + | ''i<sup>S</sup>i<sup>-</sup>'' | ||
| + | | | ||
| + | unable to utilize lactose | ||
| + | | | ||
| + | Expression | ||
| + | | | ||
| + | restore the ''i<sup>S</sup>'' phenotype in 14 revertants- giving rise to dominant ''lac<sup>-</sup>'' diploids in ''i<sup>+</sup>su<sup>+1</sup>'' or ''su<sup>+3</sup>'' | ||
| + | | | ||
| + | Plating Assay | ||
| + | | | ||
| + | in text, pg. 300-301 | ||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | ''lac<sup>+</sup> su<sup>+3</sup>'' /F''i<sup>S</sup>i<sup>-774</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup>'' | ||
| + | | | ||
| + | ''i<sup>S</sup>i<sup>-774</sup>'' | ||
| + | | | ||
| + | inducible expression of beta-galactosidase | ||
| + | | | ||
| + | Expression | ||
| + | | | ||
| + | ''i<sup>S</sup>i<sup>-774</sup> repressor, ''when suppressed by ''su<sup>+3</sup>'', becomes inducible | ||
| + | | | ||
| + | Biochemical Assay | ||
| + | | | ||
| + | Table 2- beta-galactosidase assay | ||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | ''lac<sup>+</sup> su<sup>+</sup>'' /F''i<sup>S</sup>i<sup>-</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup>'' | ||
| + | | | ||
| + | ''i<sup>S</sup>i<sup>-1058</sup>'' | ||
| + | | | ||
| + | unable to utilize lactose | ||
| + | | | ||
| + | Expression | ||
| + | | | ||
| + | dramatically suppressed by both su<sup>+1</sup>'' and ''su<sup>+3</sup>'' | ||
| + | | | ||
| + | Biochemical Assay | ||
| + | | | ||
| + | Table 2- beta-galactosidase assay | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
Revision as of 18:12, 16 December 2010
| Citation |
Bourgeois, S, Cohn, M and Orgel, LE (1965) Suppression of and complementation among mutants of the regulatory gene of the lactose operon of Escherichia coli.J. Mol. Biol. 14:300-2 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Escherichia coli; Genes; Genetic Code; Lactose; Molecular Biology; Mutation |
| edit table |
Main Points of the Paper
- The regulatory gene, i, codes for a protein whereas the operator gene, o, probably does not
Materials and Methods Used
- Parent Strain: Δlac su-/ FiSo+z+y+
- iS- dominant over i+ and not inducible by lactose sz 43
- 5 strains with suppressor mutations
- su-
- su+1
- su+2
- su+3
- su+4
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
Δlac su- /FiSo+z+y+ |
lacIS |
unable to utilize lactose |
Expression |
dominant over i+ and not inducible by lactose |
||
|
Escherichia coli |
NCBI:562 |
Δlac su- /FiSi-o+z+y+ |
lacIS |
able to utilize lactose |
Expression |
975 lac+ revertants |
||
|
Escherichia coli |
NCBI:562 |
Δlac su- /FiSoCo+z+y+ |
lacOC |
able to utilize lactose |
Expression |
125 lac+ revertants |
||
|
Escherichia coli |
NCBI:562 |
lac+ su+1 /FiSi-o+z+y+ |
iSi- |
unable to utilize lactose |
Expression |
restore the iS phenotype to 10 revertants- giving rise to dominant lac- diploids in i+su+1 |
Plating Assay |
in text, pg. 300 |
|
Escherichia coli |
NCBI:562 |
lac+ su+ /FiSi-o+z+y+ |
iSi- |
unable to utilize lactose |
Expression |
restore the iS phenotype in 14 revertants- giving rise to dominant lac- diploids in i+su+1 or su+3 |
Plating Assay |
in text, pg. 300-301 |
|
Escherichia coli |
NCBI:562 |
lac+ su+3 /FiSi-774o+z+y+ |
iSi-774 |
inducible expression of beta-galactosidase |
Expression |
iSi-774 repressor, when suppressed by su+3, becomes inducible |
Biochemical Assay |
Table 2- beta-galactosidase assay |
|
Escherichia coli |
NCBI:562 |
lac+ su+ /FiSi-o+z+y+ |
iSi-1058 |
unable to utilize lactose |
Expression |
dramatically suppressed by both su+1 and su+3 |
Biochemical Assay |
Table 2- beta-galactosidase assay |
| edit table |
</protect>
