Difference between revisions of "PMID:7007354"
(→Materials and Methods Used) |
(→Materials and Methods Used) |
||
| Line 39: | Line 39: | ||
== Materials and Methods Used == | == Materials and Methods Used == | ||
{{LitMaterials}} | {{LitMaterials}} | ||
| − | * Conditional | + | * Conditional Integrative Suppression |
| + | * This paper includes an extensive strain list | ||
| + | * P1 transduction | ||
| + | * Localized mutagenesis of the ''dnaA'' region (described elsewhere) | ||
| + | *lambda<sup>21</sup> ''dnaA'' Spi<sup>-</sup> specialized transducing phage | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 16:03, 10 April 2013
| Citation |
Schaus, N, O'Day, K, Peters, W and Wright, A (1981) Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J. Bacteriol. 145:904-13 |
|---|---|
| Abstract |
Amber mutants with defects in the dnaA gene of Escherichia coli K-12 were isolated after localized mutagenesis of the tna-dnaA region of the chromosome. We isolated 36 mutants defective in the initiation of deoxyribonucleic acid replication as determined by their dependence upon integrative suppression by a P2 sig5 prophage. Three of the 36 mutants were shown to contain amber mutations through the use of a temperature-sensitive amber suppressor. These mutations, which mapped between gyrB and tna, were characterized genetically and biochemically as amber mutations in dnaA. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Chromosome Mapping; DNA Replication; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes; Genetic Complementation Test; Mutation; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
- 3 amber mutations in dnaA were isolated and characterized
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- Conditional Integrative Suppression
- This paper includes an extensive strain list
- P1 transduction
- Localized mutagenesis of the dnaA region (described elsewhere)
- lambda21 dnaA Spi- specialized transducing phage
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
