PMID:7007354

Citation	Schaus, N, O'Day, K, Peters, W and Wright, A (1981) Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J. Bacteriol. 145:904-13
Abstract	Amber mutants with defects in the dnaA gene of Escherichia coli K-12 were isolated after localized mutagenesis of the tna-dnaA region of the chromosome. We isolated 36 mutants defective in the initiation of deoxyribonucleic acid replication as determined by their dependence upon integrative suppression by a P2 sig5 prophage. Three of the 36 mutants were shown to contain amber mutations through the use of a temperature-sensitive amber suppressor. These mutations, which mapped between gyrB and tna, were characterized genetically and biochemically as amber mutations in dnaA.
Links	PubMed PMC217197
Keywords	Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Chromosome Mapping; DNA Replication; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes; Genetic Complementation Test; Mutation; Transduction, Genetic
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Main Points of the Paper

Please summarize the main points of the paper.

• 3 amber mutations in dnaA were isolated and characterized

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

• Conditional Integrative Suppression
• This paper includes an extensive strain list
• P1 transduction
• Localized mutagenesis of the dnaA region (described elsewhere)
• lambda²¹ dnaA Spi^- specialized transducing phage

Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NS473 NCBI Taxon ID: 83333	Genotype of Reference Strain: dnaA+ Genotype of Experimental Strain : dnaA311(Am)	Reference Condition:	0000271	temperature-dependent growth	0000178	in vivo assay evidence	reduced rate of DNA synthesis in high temperature. See figure 4A for experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NS473 NCBI Taxon ID: 83333	Genotype of Reference Strain: dnaA+ Genotype of Experimental Strain : dnaA366(Am)	Reference Condition:	0000271	temperature-dependent growth	0000178	in vivo assay evidence	reduced rate of DNA synthesis in high temperature. See figure 4B for experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NS473 NCBI Taxon ID: 83333	Genotype of Reference Strain: dnaA+ Genotype of Experimental Strain : dnaA367(Am)	Reference Condition:	0000271	temperature-dependent growth	0000178	in vivo assay evidence	reduced rate of DNA synthesis in high temperature. See figure 4C for experimental results.
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References

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