Difference between revisions of "PMID:4933690"

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'''Wilson, TH, Kusch, M and Kashket, ER'''  (1970) A mutant in Escherichia coli energy-uncoupled for lactose transporta defect in the lactose-operon.''Biochem. Biophys. Res. Commun.'' '''40''':1409-14
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'''No author listed'''  (1970) A mutant in Escherichia coli energy-uncoupled for lactose transporta defect in the lactose-operon. ''Biochem. Biophys. Res. Commun.'' '''40''':1409-14
 
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!align=left |Abstract
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No abstract in PubMed
 
No abstract in PubMed
 
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4933690 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4933690 PubMed]
  
 
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!align=left |Keywords
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Biological Transport, Active; Carbon Isotopes; Cell Membrane; Coliphages; Escherichia coli; Galactose; Glycosides; Lactose; Molecular Biology; Mutation; Nitrobenzenes; Operon; Transduction, Genetic
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Biological Transport, Active; Carbon Isotopes; Cell Membrane/metabolism; Coliphages; Escherichia coli/metabolism; Galactose/metabolism; Glycosides/metabolism; Lactose/metabolism; Molecular Biology; Mutation; Nitrobenzenes; Operon; Transduction, Genetic
  
 
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==Main Points of the Paper ==
 
==Main Points of the Paper ==
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*Isolation of mutant (X71-54) with a severe defect in TMG accumulation
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{{LitSignificance}}
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**possesses elevated membrane carrier activity, therefore the rate of efflux is increased
 
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
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*Parent Strain: X71 (''i<sup>-</sup>z<sup>+</sup>y<sup>+</sup>a<sup>-</sup>, proC<sup>-</sup>, try<sup>-</sup>'', B<sub>1</sub><sup>-</sup>, F<sup>-</sup>)
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*Additional Strain: X71-54
 
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*Additional Strain: Ca77 (lactose-deleted, B<sub>1</sub><sup>-</sup>, Hfr)
 
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*β-galactosidase assay
 
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*ONPG uptake assay
 
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*Thiomethylgalactoside (TMG) transport assay
 
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*P<sub>1</sub> Transduction
 
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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''Escherichia coli''
 
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NCBI:83333
 
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X71-54
 
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''lacY<sup>UN</sup>''
 
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*Decreased ability to maintain concentration gradient?
 
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*Abolished energy-coupling transporter activity?
 
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*Absent intracellular accumulation of thiomethylgalactoside (direct observation)
 
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Other
 
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Intracellular [<sup>14</sup>C]TMG accumulation
 
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Other
 
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Figure 1- Filter assay
 
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''Escherichia coli''
 
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NCBI:83333
 
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X71-54
 
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''lacY<sup>UN</sup>''
 
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*Decreased intracellular accumulation of substance
 
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*Increased rate of transporter efflux?
 
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*Decreased energy-coupled transport?
 
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*Increased rate of efflux of thiomethylgalactoside (TMG) (direct observation)
 
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Other
 
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exit rate of [<sup>14</sup>C]TMG was faster than that found for the parent
 
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Other
 
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Figure 2- Filter assay
 
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''Escherichia coli''
 
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NCBI:83333
 
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X71-54
 
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''lacY<sup>UN</sup>''
 
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*Increased rate of transporter substrate uptake?
 
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*Increased transport of small molecules
 
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*Increased rate of transporter activity
 
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Metabolic Activity
 
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130% of normal uptake activity (gauged by ONPG entry)
 
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Biochemical Assay
 
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Table 1- ONPG assay
 
  
 
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==Notes==
 
==Notes==
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Additional papers regarding this mutant can be found [[PMID:4577939|here]]
 
  
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==References==
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[[Category:Publication]]
 
[[Category:Publication]]
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Latest revision as of 13:16, 1 September 2013

Citation

No author listed (1970) A mutant in Escherichia coli energy-uncoupled for lactose transporta defect in the lactose-operon. Biochem. Biophys. Res. Commun. 40:1409-14

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Biological Transport, Active; Carbon Isotopes; Cell Membrane/metabolism; Coliphages; Escherichia coli/metabolism; Galactose/metabolism; Glycosides/metabolism; Lactose/metabolism; Molecular Biology; Mutation; Nitrobenzenes; Operon; Transduction, Genetic

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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