Difference between revisions of "PMID:5330222"

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'''Müller-Hill, B'''  (1966) Suppressible regulator constitutive mutants of the lactose system in Escherichia coli.''J. Mol. Biol.'' '''15''':374-6
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'''No author listed'''  (1966) Suppressible regulator constitutive mutants of the lactose system in Escherichia coli. ''J. Mol. Biol.'' '''15''':374-6
 
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!align=left |Abstract
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No abstract in PubMed
 
No abstract in PubMed
 
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=5330222 PubMed]
 
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=5330222 PubMed]
  
 
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!align=left |Keywords
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Enzyme Repression; Escherichia coli; Galactosidases; Genetic Code; Guanidines; Lactose; Molecular Biology; Mutation; Purines; RNA, Messenger
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Enzyme Repression; Escherichia coli/metabolism; Galactosidases/biosynthesis; Genetic Code; Guanidines/pharmacology; Lactose/metabolism; Molecular Biology; Mutation; Purines/pharmacology; RNA, Messenger/metabolism
  
 
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==Main Points of the Paper ==
 
==Main Points of the Paper ==
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*Suppressors of nonsense codons exist for the regulatory ''i'' gene of the lactose operon, thereby proving that the functional i product is a protein.
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{{LitSignificance}}
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**Rationale: suppression of nonsense codons into amino acids occurs at the translational level
 
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*Specific activity of β-galactosidase in the wild type is 12; in both the suppressible (but unsuppressed) constitutives 18,000.
 
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**values in a large number of different ''su+'' revertants from these two constitutive strains range from 600 to 2000, indicating incomplete suppression and the existence of different suppressors.
 
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
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*Parent Strain: 112-12 (''gal<sup>-</sup>cys<sup>-</sup>his<sup>-</sup>su<sup>-</sup>'')
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{{LitMaterials}}
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**''gal<sup>-</sup>'' and ''cys<sup>-</sup>''mutations are suppressible by the same suppressor(s)
 
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* Mutagenesis using 2-aminopurine (2AP) or N-methyl-N'-nitro-N-nitrosguanidine
 
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*ONPG solution to select for ''lac'' constitutive mutants
 
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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''Escherichia coli''
 
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NCBI:562
 
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112-12''lacI<sup>-</sup><sup>(c)</sup>''
 
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''su''
 
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*Growth phenotype on medium
 
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*Positive suppression
 
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Growth
 
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colonies on D-galactose + L-histidine plates (revertants to ''su<sup>+</sup>'')
 
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Plating Assay
 
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in text, pg. 374
 
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''Escherichia coli''
 
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NCBI:562
 
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112-12''lacI<sup>-</sup><sup>(c)</sup>''
 
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''lacI''
 
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*Decreased beta-galactosidase expression
 
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Expression
 
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decreased beta-galactosidase level (nonsense mutation present in ''lacI'' gets suppressed in the presence of ''su<sup>+</sup>'')
 
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Biochemical Assay
 
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in text, pg. 374
 
  
 
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==Notes==
 
==Notes==
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==References==
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[[Category:Publication]]
 
[[Category:Publication]]
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Latest revision as of 13:16, 1 September 2013

Citation

No author listed (1966) Suppressible regulator constitutive mutants of the lactose system in Escherichia coli. J. Mol. Biol. 15:374-6

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Enzyme Repression; Escherichia coli/metabolism; Galactosidases/biosynthesis; Genetic Code; Guanidines/pharmacology; Lactose/metabolism; Molecular Biology; Mutation; Purines/pharmacology; RNA, Messenger/metabolism

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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